包虫囊液对体外培养小鼠NK细胞影响的研究  

The Pilot Study on the Influence of NK Cell From Mouse Spleen Cells Treated with Echinococcus Granulosus Fluid in Vitro

作　　者：印双红[1] 陈小林[2] 张俊波[3] 吴向未[2] 陈雪玲[2] 

机构地区：[1]铜仁学院大健康学院,贵州铜仁554300 [2]石河子大学医学院免疫学教研室,新疆石河子832002 [3]铜仁学院农林工程与规划学院,贵州铜仁554300

出　　处：《铜仁学院学报》2016年第4期1-4,共4页Journal of Tongren University

基　　金：国家自然科学基金项目(No.81360453);贵州省科技合作计划项目(黔科合LH字[2015]7238号;黔科合LH字[2015]7236号)

摘　　要：采用流式细胞仪检测NK细胞活性受体NKG2D的表达变化,乳酸脱氢酶细胞毒性检测法(LDH)分析脾细胞的杀伤活性。通过细粒棘球蚴囊液对体外BABL/c小鼠NK细胞的检测结果的分析讨论,得到细粒棘球蚴囊液可降低NKG2D的表达水平,进而使NK细胞杀伤活性降低,从而得到有利于包虫免疫逃逸的结论。To observe the influence of NK cells which separated from mouse spleen cells treated with Echinococcus granulosus fluid in vitro. The spleen cells of BABL/C mouse were co-cultured with different concentration Hydatid fluid. The activity and expression of NKG2 D were detected by FCM; and the killing activity of spleen cells were also detected by LDH assay. The result shows that with the increasing of the concentration of Hydatid fluid, the expression of NKG2 D and NK cell activity were decreased. The expression of NKG2 D was decreased, thus reducing the activity of NK cells, which may facilitate Echinococcus granulosus to evade the host＇s immune.

关 键 词：囊液 细粒棘球蚴 NK细胞 

分 类 号：R392[医药卫生—免疫学]