大鳞鲃生长、血清性激素水平和性腺发育的研究  

Growth,sexual hormone levels in serum and gonadal development in barbell Luciobarbus capito

作　　者：王四泽 张颖[1] 耿龙武[1] 姜海峰[1] 徐伟[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《大连海洋大学学报》2016年第4期351-356,共6页Journal of Dalian Ocean University

基　　金：国家"十二五"科技支撑计划项目(2012BAD25B09);农业科技成果转化资金资助项目(2013GB23260584);中央级公益性科研院所基本科研业务费专项(HSY201403);哈尔滨市科技攻关计划项目(2012AA6CN037)

摘　　要：为掌握大鳞鲃Luciobarbus capito F1代生长、血清性激素变化、性腺发育规律和后备亲鱼的初次性成熟时间,采用形态学、组织学和生物化学方法,对养殖条件下1-5龄大鳞鲃的生长特点、血清性激素含量和性腺发育进行了研究。结果表明：随着年龄的增长,大鳞鲃体质量和体长显著增加（P〈0.05）,体长与体质量的关系式为W=0.012L2.99（R^2=0.99）,Von Bertalanffy生长方程为Lt=107.43[1-e-0.15（t＋0.067）]、Wt=12939[1-e-0.15（t＋0.067）]2.99;1-5龄雄鱼血清中雌二醇（17β-estradiol,17β-E2）含量均低于雌鱼,1-4龄雌鱼血清中17β-E2含量随年龄的增加而升高,在4龄时达到最高值,为（713.00±424.89）pg/m L;5龄雄鱼血清中的睾丸酮（Testosterone,T）含量最高,为（1.12±0.81）ng/m L,其余各龄组血清中T含量无显著性差异（P〉0.05）;大鳞鲃精巢为典型的小叶型结构,精小叶间有间质细胞,小叶内有支持细胞及精小囊结构,精子发育可分为精原细胞、初级精母细胞、次级精母细胞、精子细胞、成熟精子5个时相,5龄雄鱼成熟系数（GSI）达到最大值（0.83%）;大鳞鲃卵巢中卵母细胞发育可分5个时相,GSI为0.51%-8.90%,5龄雌鱼成熟系数最大（8.90%）。研究表明,大鳞鲃雌鱼性腺4龄时部分成熟,5龄时完全成熟,雄鱼性腺比雌鱼早成熟一年,在人工繁殖过程中,大鳞鲃后备亲鱼在雄鱼4龄、雌鱼5龄时适合人工药物催产。The growth,gonadal development and serum sex steroids level in 1-5 years old cultured barbel Luciobarbus capito were investigated using morphological,histological and biochemical methods to study the growth characteristics,and relationship between gonadal development and sex steroids level.The results showed that body length（L） and body weight（W） were shown to increased significantly with age,with body length-weight relationship as W = 0.012L2.99（R^2= 0.99）,and Von Bertalanffy growth equation Lt= 107.43[1-e-0.15（t＋0.067）] and Wt=12939[1-e^-0.15（t＋0.067）]2.99.There was lower level of serum testosterone（T） in 1-5 years old males than that in 1-4years old females,with the maximal value（1.12±0.81） ng / m L at age of 5 years old.The maximal level（713.00 pg / m L±424.89 pg / m L） of 17β-E2 was observed in 5 years old females,without significant difference among the other groups.The Leydigs cells,sertolis cells and spermatangia were distributed in the seminiferous lobules of the testis as a typical lobular structure.Spermatic development was divided into five stages,spermatogonium,first spermatocyte,secondary spermatocyte,spermatoblast and mature sperms,with the maximal gonado-somatic index（GSI）（0.83%） in 5 years old males.The ovary development was divided into five stages,with GSI from 0.51% to8.90%,and the peak value of 8.90% at stage Ⅴ,one year early maturation in male than in female,4 years old at maturity in male and 5 years old at maturity in female.

关 键 词：大鳞鲃 生长 睾丸酮 雌二醇 性腺发育 

分 类 号：S917.4[农业科学—水产科学]