四川大豆疫霉毒力鉴定及资源抗性分析  

Pathotypes identification of Phytophthora sojae in Sichuan Province and resistance analyses of soybean germplasm

作　　者：陈四维[1] 张婷婷[1] 肖丽[1] 武晓玲[1] 杨文钰[1] 

机构地区：[1]四川农业大学农学院,四川成都611130

出　　处：《中国油料作物学报》2016年第4期539-542,共4页Chinese Journal of Oil Crop Sciences

基　　金：现代农业产业技术体系专项(CARS-04-PS19);四川省科技厅项目(2015NZ0040)

摘　　要：采用土壤诱捕和组织分离的方法,从四川省仁寿县和雅安市大豆根腐病发病田块采集的土壤中和病株上分离到4个大豆疫霉分离物RS1、RS2和YA1、YA2,用含有已知抗性基因的鉴别寄主进行毒力鉴定。结果表明YA1和YA2毒力公式相同,为1a,1b,1d,2,3c,属于同一致病型;RS1、RS2毒力公式分别为1a,1d,1k,3a,3c,4,5和1a,1b,1c,1d,1k,2,3c,5,分别为不同致病型。用3个致病型接种157份大豆资源,结果表明,抗病种质相对较少,占23.57%;地方品种抗病性较育成品种强,本研究可为大豆抗病性育种提供科学依据。Phytophthora root rot of soybean seriously damaged the soybean production in Renshou and Yaan of Sichuan Province. Four isolates of Phytophthora sojae（ RS1,RS2,YA1,YA2） were isolated by using baiting method from soil and tissue isolated from diseased plants. Their virulence determination was carried out on 11 differentials host containing known resistant genes. Results showed that YA1 and YA2 had the same virulence formula of 1a,1b,1d,2,3c,indicating their same pathotype. The virulence formula of RS1 and RS2 were 1a,1d,1k,3a,3c,4,5and 1a,1b,1c,1d,1k,2,3c,5,respectively. 157 soybean germplasm were inoculated with 3 pathotypes of P. sojae.Results showed that local varieties had more resistance than cultivated variety,however the amount of resistant germplasms accounted for only 23. 57%. The research could provide useful information for resistant breeding.

关 键 词：大豆 疫霉根腐病 致病型 毒力公式 抗性分析 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]