黄芪多糖对小鼠卵母细胞体外成熟的影响  

Effects of Astragalus Polysaccharides on in Vitro Maturation of Mouse Oocytes

作　　者：闫学花[1] 王丽丽[1] 张锐[1] 文杨[1] 李科瑛 杨萌[1] 雷安民[1] 张为民[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《动物医学进展》2016年第9期79-83,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31172280)

摘　　要：采集未成熟小鼠卵母细胞添加不同浓度的黄芪多糖(APS)进行体外成熟(IVM)培养,统计卵母细胞生发泡破裂(GVBD)发生率以及第一极体(first polarbody,PB1)排出率,并通过卵母细胞JC-1染色方法进行线粒体膜电位检测;进一步在卵母细胞培养液中添加丙酮醛(MG)与黄芪多糖共培养,统计GVBD发生率及PB1排出率,检测活性氧(ROS)产生情况,探讨黄芪多糖在小鼠卵母细胞体外培养及体外成熟中的作用。结果显示,黄芪多糖对小鼠卵母细胞体外成熟,包括GVBD发生率和PB1排出率无显著影响。添加丙酮醛与黄芪多糖共培养结果显示,10mg/mL+APS+MG试验组PB1排出率显著降低,相应的卵母细胞ROS产生量显著升高,但对GVBD发生率则无显著影响。In order to explore the effect of Astragalus polysaccharides（APS） in the process of in vitro maturation （IVM） on mouse oocytes, immature mouse oocytes were collected from 6-8 week old mice and cultured in vitro with APS（ 0.1 mg/mL, 1.0 mg/mL, 10 mg/mL）, and the rates of germinal vesicle breakdown （GVBD） and first polar body （PB1） elimination of oocytes were counted by statistics. The mitochondrial membrane potential in each group was detected. Simultaneously, the mouse oocytes were cultured with methylglyoxal （MG） and APS,and the production of ROS （reactive oxygen species） was detection. The results showed that addition of APS had no significant effects on mouse oocytes＂ maturation,including the rates of GVBD and PBE1. The result of addition of APS and MG for coculture of oocytes showed that the rate of PB1 elimination was significantly reduced in the group MG＋10 mg/mL APS,and the ROS contents of oocyts increased significantly,but had no significant effect on GVBD rate.

关 键 词：小鼠卵母细胞 黄芪多糖 体外培养 

分 类 号：S852.165[农业科学—基础兽医学]