白沙绿茶提取物对原发性高血压大鼠降压效果的研究  

Effect of Baisha greentea extract on blood pressure in treating spontaneous hypertension rats

作　　者：虞道锐[1] 姬立平 何以红 周晶[3] 吴常艳[3] 刘启兵[4] 

机构地区：[1]海南医学院基础与生命科学学院基础医学研究中心,海南海口571199 [2]西安交通大学药理学系,陕西西安710049 [3]海南医学院临床学院,海南海口571199 [4]海南医学院药学院药理学教研室,海南海口571199

出　　处：《海南医学院学报》2016年第18期2077-2081,共5页Journal of Hainan Medical University

基　　金：海南省中药现代化项目(ZY201426);海南省重点科技计划项目(ZDXM2014070)~~

摘　　要：目的:探讨白沙绿茶提取物对原发性高血压大鼠(SHR)的降压效果及其机制。方法:将60只原发性高血压大鼠(SHR)随机分为高血压对照组,苯磺酸氨氯地平组和白沙绿茶提取物高、中、低剂量组,共5组,每组12只。每日给予相应药物灌胃,对照组给予等量蒸馏水。每周对各组大鼠血压测量1次;7周末,自腹主动脉取血,用于测定血清一氧化氮(NO)、超氧化物歧化酶(SOD)活性以及血管紧张素Ⅱ(AngⅡ)含量,同时取心脏组织HE染色后观察病理组织形态学的变化。结果:与高血压对照组相比,白沙绿茶提取物三个剂量组与氨氯地平组均对大鼠的血压有一定的降低趋势,差异具有统计学意义(P<0.01),白沙绿茶提取物高(200 mg·kg^(-1)·d^(-1))、中(100 mg·kg^(-1)·d^(-1))剂量组和氨氯地平组(0.5 mg·kg^(-1)·d^(-1))可显著升高血清NO和SOD含量,差异均具有统计学意义(P<0.05或P<0.01)。结论:白沙绿茶提取物可升高SHR大鼠血清NO和SOD水平以及降低血管紧张素Ⅱ(AngⅡ)含量,产生降压效应,同时能有效逆转高血压大鼠病理性心肌重构。Objective： To explore the effect of Baisha greentea extract on blood pressure in treating spontaneous hypertension rats. Methods： A total of 60 primary hypertensive rats（ SHR） were randomly divided into hypertension of control group,amlodipine besylate group and the high,medium and low dose group of green tea extract of five groups,with twelve rats in each group. All treatments were given through oral,and the control group with distilled water. Each measurement of rat blood pressure was detected in themorning every week. After 7 weeks treatment,blood was collected from the rat abdominal aorta for determination of serum nitric oxide（ NO） and superoxide dismutase（ SOD） activity and level of plasma angiotensin II. At the same time,heart tissue was embedded in10% neutral buffered formaldehyde fixation,paraffin and sectioned. Then the changes of histopathological were changed after HE staining. Results： Compared with the SHR control group,systolic and diastolic blood pressure of essential hypertension in high dose group and middle dose group and amlodipine group was significantly reduced. At the same time,the NO and SOD contents of Baisha greentea extract in high dose group（ 200 mg·kg-（-1）·d-（-1）） and middle dose group（ 100 mg·kg-（-1）·d-（-1）） and amlodipine group（ 0. 5 mg·kg-（-1）·d-（-1）） was significantly increased（ P〈0. 05 and P〈0. 01）. Conclusion： Long-term ingestion of Baisha greentea extract could significantly elevated serum NO and SOD levels of SHR,reduce the level of plasma angiotensin Ⅱ to common level,while reverse the hypertensive rat myocardial remodeling effectively.

关 键 词：白沙绿茶提取物 原发性高血压大鼠 降压 血管紧张素Ⅱ 

分 类 号：R544.1[医药卫生—心血管疾病]