特发性膜性肾病患者血清和肾组织中PLA2R、Nephrin的检测及其与病情的相关性  

Detection of PLA2R and Nephrin in serum and kidness tissue of patients with idiopathic membranous nephropathy and their correlation with the illness

作　　者：及臻臻 管海玉 李成杰[1] 宣亚男 

机构地区：[1]南方医科大学中西医结合医院肾病科,广东广州510315

出　　处：《海南医学院学报》2016年第18期2139-2142,共4页Journal of Hainan Medical University

基　　金：广东省科技计划项目(2014A020208801)~~

摘　　要：目的:研究特发性膜性肾病患者血清及肾组织中PLA2R及Nephrin含量与病情的相关性。方法:选择特发性膜性肾病(IMN)患者、继发性膜性肾病(SMN)患者以及健康体检者,分别作为IMN组、SMN组以及对照组,采集三组受试者的血清并测定PLA2R、Nephrin含量及肾功能指标;采集IMN患者的肾组织并测定PLA2R、Nephrin含量,采集IMN患者的尿液并测定C5b-9、PCX的含量。结果:IMN组患者的血清PLA2R水平显著高于SMN组和对照组、Nephrin水平显著低于SMN组和对照组,SMN组患者的血清PLA2R水平显著高于对照组、Nephrin水平显著低于对照组;IMN组肾脏组织中PLA2R的染色强度及含量均高于SMN组、Nephrin的染色强度及含量均低于SMN组;IMN组中PLA2R阳性患者的血清BUN、CREA含量以及尿液C5b-9、PCX含量均显著高于PLA2R阴性患者,血清ALB含量显著低于PLA2R阴性患者;IMN组中Nephrin阳性患者的血清BUN、CREA含量以及尿液C5b-9、PCX含量均显著低于Nephrin阴性患者,血清ALB含量显著高于Nephrin阴性患者。结论:特发性膜性肾病患者血清及肾组织中PLA2R含量升高、Nephrin降低,PLA2R及Nephrin含量改变与肾功能损伤、足细胞损伤相关。Objective： To study the expression of PLA2 R and Nephrin levels in serum and kidney tissue of patients with idiopathic membranous nephropathy and their correlation with the illness. Methods： Patients with idiopathic membranous nephropathy（ IMN）,patients with secondary membranous nephropathy（ SMN） and healthy subjects were selected and enrolled in IMN group,SMN group and control group respectively. Serum was collected from three groups to determine PLA2 R and Nephrin levels and renal function indexes,kidney tissue was collected from patients with IMN patients to determine PLA2 R and Nephrin levels,and urine was collected from patient with IMN to determine C5b-9 and PCX levels. Results： Serum PLA2 R level of IMN group was significantly higher than that of SMN group and control group while Nephrin level was significantly lower than that of SMN group and control group,and serum PLA2 R level of SMN group was significantly higher than that of control group while Nephrin level was significantly lower than that of control group; the staining intensity and level of PLA2 R in kidney tissue of IMN group were higher than those of SMN group while thestaining intensity and level of Nephrin were lower than those of SMN group; BUN and CREA levels in serum as well as C5b-9 and PCX levels in urine of IMN patients with positive PLA2 R were significantly higher than those of patients with negative PLA2 R,and serum ALB level was significantly lower than that of patients with negative PLA2R; BUN and CREA levels in serum as well as C5b-9 and PCX levels in urine of IMN patients with positive Nephrin were significantly lower than those of patients with negative Nephrin,and serum ALB level was significantly higher than that of patients with negative Nephrin. Conclusions： PLA2 R levels increase and Nephrin levels decrease in serum and kidney tissue of patients with idiopathic membranous nephropathy,and the changes of PLA2 R and Nephrin levels are associated with renal function damage and podocyte damage.

关 键 词：特发性膜性肾病 M型磷脂酶A2受体 NEPHRIN 足细胞 

分 类 号：R692.3[医药卫生—泌尿科学]