同时接种伪狂犬病活疫苗Bartha-K61株对嵌合载体猪瘟疫苗rAdV-SFV-E2免疫效力的影响  

Effects of co-administered pseudorabies Bartha-K61 vaccine on the efficacy of an adenovirus/alphavirus replicon chimeric vectored vaccine rAdV-SFV-E2

作　　者：夏水利 孙元[2] 雷建林[2] 杜明亮[2] 丛鑫[2] 杜恩岐[1] 仇华吉[2] 

机构地区：[1]西北农林科技大学动物医学学院,陕西杨凌712100 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2016年第8期642-645,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863计划(2011AA10A208)

摘　　要：本实验室前期研究中构建了一种新型的猪瘟标记疫苗—腺病毒/甲病毒复制子嵌合载体猪瘟疫苗(rAdV-SFV-E2),并证实该疫苗具有与C株猪瘟疫苗相同的免疫效力。为评价该疫苗与伪狂犬病疫苗(Bartha-K61)联合免疫是否存在相互干扰现象,本研究将这两种疫苗联合免疫健康仔猪(n=5),同时设立单独免疫对照和阴性对照,免疫后利用CSFV强毒石门株进行攻击,并通过抗体反应、临床症状、病毒血症、病理学及组织病理学变化等指标对疫苗免疫效果进行评价。结果显示,rAdV-SFV-E2和Bartha-K61联合免疫后与单独免疫rAdV-SFV-E2均能够对致死性CSFV强毒的攻击提供完全保护(5/5存活),表现为无猪瘟特异性临床症状、无病毒血症、无病理变化和组织病理学变化。该研究证实,rAdV-SFV-E2和伪狂犬病疫苗Bartha-K61同时免疫对rAdV-SFV-E2的免疫效果无干扰现象。In a previous study, we showed that an adenovirus-delivered, Semliki forest virus replicon-vectored vaccine （rAdV-SFV-E2） against CSFV was able to protect pigs against lethal classical swine fever virus （CSFV） challenge. In order to evaluate the efficacy of rAdV-SFV-E2 co-administered with pseudorabies vaccine Bartha-K61, four groups of 5-week-old pigs （n=5） were immunized intramuscularly twice with either 107 TCID50 rAdV-SFV-E2 or one dose of Bartha-K61 alone or 107 TCIDs0 rAdV-SFV-E2 combined with one dose of Bartha-K61 at an interval of three weeks, and challenged with the virulent CSFV Shimen strain at four weeks post-booster immunization. The results showed that all the pigs co-immunized with rAdV-SFV-E2 and Bartha-K61 developed high-level specific antibodies against CSFV and PRV comparable to either vaccine administered alone and fully protected the immunized pigs against lethal CSFV challenge. These data demonstrated that Bartha-K61 has no interference with the efficacy of rAdV-SFV-E2.

关 键 词：猪瘟 嵌合载体疫苗 伪狂犬病疫苗 联合免疫 评价 

分 类 号：S852.65[农业科学—基础兽医学]