白细胞介素8对高糖诱导的人脂肪间充质干细胞损伤的保护作用  

Protective effect of interleukin-8 on injury of human adipose derived mesenchymal stem cells induced by high glucose

作　　者：沈雷[1] 沙峰[1] 孙权[1] 张鹏[1] 孙石柱[1] 张晓东[1] 姚立杰[1] 李静平[1] 

机构地区：[1]齐齐哈尔医学院解剖教研室,黑龙江齐齐哈尔161006

出　　处：《中国现代医学杂志》2016年第16期18-22,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金项目(No:81541137);黑龙江省教育厅指令科研课题(No:12541901)

摘　　要：目的探讨白细胞介素8(IL-8)对高糖诱导人脂肪间充质干细胞(h Ad MSC)损伤的保护作用。方法含250 mmol/L葡萄糖的培养基建立细胞高糖模型;P65质粒转染人IL-8基因到h Ad MSC为IL-8转染组,仅转染P65质粒者为P65对照组,在IL-8转染组中添加PD98059为Erk抑制剂组。利用MTT细胞增殖实验、ELISA或流式细胞技术检测各组h Ad MSC增殖的光密度值(OD值)、Caspase-3、Erk或VEGF等蛋白的表达。结果与P65对照组比较,IL-8转染组h Ad MSC增殖OD值明显升高,Caspase-3蛋白表达下降(P<0.01);IL-8转染组Erk蛋白活性和VEGF蛋白含量明显高于P65对照组(P<0.01);但与IL-8转染组比较,Erk抑制剂组h Ad MSC增殖的OD值降低,Caspase-3蛋白表达升高(P<0.01)。结论 IL-8在高糖环境下,通过Erk通路,发挥对h Ad MSC的保护作用。Objective To investigate the protective effect of interleukin-8 （IL-8） on high glucose induced injury of human adipose derived mesenchymal stem cells （hAdMSC） induced by high glucose. Methods High glucose model with 250 mmol/1 glucose medium was established. In IL-8 transfection group, human IL-8 gene was transfected into hAdMSC by p65 plasmid. In the control group, and P65 plasmid was transfected, and in Erk Inhibitor group, PD98059 was added into IL-8 transfection group. MTF cell proliferation assay, ELISA and flow cytometry was used to detect hAdMSC proliferation of the optical density （OD） or Caspase-3, Erk and VEGF protein. Results Compared with the p65 control group, OD of IL-8 transfection group was increased significantly and the proliferation of hAdMSC was increased, Caspase-3 protein expression was decreased, P 〈 0.01. Erk protein activity and VEGF protein in IL- 8 transfected group were significantly higher than those in p65 control group, P 〈 0.01. But compared with the IL-8 group, OD of hAdMSC proliferation in Erk inhibitor group was decreased, expression of Caspase-3 protein was increased, P 〈 0.01. Conclusions In the high glucose environment, IL-8 plays a protective role for human adipose derived mesenchymal stem cells through the Erk signal pathway.

关 键 词：高糖 白细胞介素8 人脂肪间充质干细胞 ERK通路 

分 类 号：R641[医药卫生—外科学]