五味子甲素衍生物联苯双酯的紫外防护作用及机制研究  

Protective effect of Sch A derivant Dimethyl dicarboxylate biphenyl against damage of UVB irradiation and its mechanism

作　　者：曹波[1] 王娟娟[2] 陈诗雅[1] 路婷婷[1] 陈虹[1] 齐莉[1] 

机构地区：[1]武警后勤学院,天津300309 [2]天津医科大学,天津300070

出　　处：《中国现代医学杂志》2016年第16期23-28,共6页China Journal of Modern Medicine

基　　金：天津市应用基础与前沿技术研究计划(青年项目)(No:14JCQNFC10300);武警后勤学院院级科学基金(No:WHM201308);国家自然基金资助项目(No:81072964)

摘　　要：目的探讨联苯双酯(DDB)对中波紫外线(UVB)的防护作用及其相关机制研究,为临床应用提供理论依据。方法 DDB和维生素C对照检测五味子甲素衍生物DDB对超氧阴离子(O_2^-)的清除率。MTT法测定DDB、UVB对人永生化角质形成细胞(Ha Ca T)、人真皮成纤维细胞(FB)生长的影响及DDB对UVB的防护作用。通过Giemsa染色,从形态学上观察DDB对UVB损伤的防护作用。激光共聚焦观察活性氧簇(ROS)及检测其荧光强度。结果实验结果表明,相同浓度DDB具有强的清除O_2^-的能力;DDB对Ha Ca T、Fb细胞无毒性且可防护UVB损伤。Giemsa染色后,形态学上发现DDB可以明显抑制细胞的凋亡。DDB可抑制Ha Ca T细胞外ROS的生成。结论五味子甲素衍生物DDB对UVB有明显的防护作用,DDB作用机制是通过其抗氧化活性抑制UVB辐射引起的氧化应激及细胞凋亡等,起到紫外线防护作用,有望开发成为有效的抗氧化剂和紫外防护产品。Objective To evaluate the protective activity of SchA derivant DDB on the UVB-induced skin damage and explore its mechanism. Methods The Anti-oxidation activity against reactive oxygen species （ROS） including superoxide anion radical （O2-） was investigated. Methyl thiazolyl tetrazolium （MTT） assay was used to examine the effect of DDB or UVB on HaCaT and Fb cells viability and its protective effect from UVB＇s damage. UVB-induced cells apoptosis was visualized with Giemsa stain. Anti-oxidative capacity of the drugs was evaluated by dichlorodi- hydrofluoreseein diaeetate （DCFH-DA） assay. Results In this study, the topical application of DDB after UVB irradiation was found to decrease UVB-induced oxidation damage, the drug had a highly activity of superoxide anion radical （O2-）. Through MTT assay, we found that DDB was found had no damage to cells. Meanwhile, we also find that DDB can decrease human melanoma cell＇s production.Through Giemsa stain, we visualized that the drug can inhibite UVB induced HaCaT cell death. Conclusions In a word, DDB may be useful in antiphotoaging agents in the treatment of UVB irradiation.

关 键 词：五味子甲素衍生物联苯双酯 机制研究 UVB 

分 类 号：R932[医药卫生—生药学]