直接灸“肝俞”对原发性肝癌癌前病变大鼠T淋巴细胞亚群的影响  

Effects of Direct Moxibustion of “Ganshu”(BL 18)on the Contents of T cells in Peripheral Blood in Rats with Precancerous Lesion of Primary Hepatocellular Carcinoma

作　　者：闫亚南[1] 王宁[1] 王卓昱 田瑶[1] 成宇镇 马惠芳[1] 侯中伟[1] 马佳佳[1] 关翰宇 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《针刺研究》2016年第4期321-326,共6页Acupuncture Research

基　　金：国家自然科学基金青年科学基金项目(No.81202760)

摘　　要：目的:观察不同灸量对原发性肝癌癌前病变模型大鼠外周血中T淋巴细胞亚群含量的影响,探讨直接灸对肝癌大鼠免疫功能的影响。方法:雄性Wistar大鼠随机分为空白组(10只)、模型组(15只)、直接灸1组(15只)、直接灸2组(15只)以及隔姜灸组(15只),采用腹腔注射二乙基亚硝胺法逐步诱导肝癌模型,为期10周。直接灸1组、直接灸2组造模的同时分别给予直接灸"肝俞"穴3壮/穴、6壮/穴干预,隔日1次;隔姜灸组造模开始的同时运用"肝俞"隔姜灸法进行干预,3壮/穴,隔日1次,连续10周。运用流式细胞仪检测大鼠外周血中T淋巴细胞亚群CD 3^+、CD 4^+、CD 8^+的含量,HE染色观察大鼠肝脏组织形态学变化。结果:与空白组比较,模型组CD 3^+、CD 4^+T细胞含量降低(P<0.05,P<0.01);直接灸干预后其含量显著升高(P<0.05,P<0.01);而隔姜灸组与模型组比,CD 3+T细胞含量差异无统计学意义(P>0.05),CD 4^+T细胞含量显著升高(P<0.01)。与空白组比较,模型组CD 8^+T细胞含量升高(P<0.01);直接灸1、2组及隔姜灸组CD 8+T细胞含量与模型组比显著降低(P<0.01)。与模型组比较,艾灸后CD 4^+/CD 8^+比值显著升高(P<0.01)。在病理改变方面,艾灸各干预组镜下癌细胞分化程度高,异型性低。结论:直接灸和隔姜灸对肝癌癌前病变病理形态学变化有改善作用,可能与调节外周血中CD 3^+、CD 4^+、CD 8^+T细胞的含量,提高充分免疫应答效应,增强免疫监视,避免肿瘤细胞逃逸相关。Objective To observe the effect of moxibustion stimulation of＂Ganshu＂（BL 18）region on contents of T cells in the peripheral blood in rats with Diethylnitrosamine（DEN）-induced hepatocellular carcinoma（HCC）,so as to explore its effective in improving immunoregulatory function.Methods Seventy male Wistar rats were randomly divided into control group（n=10）,model group（n=15）,direct moxibustion-15sgroup（n=15）,direct moxibustion-30sgroup（n=15）and ginger-separated moxibustion group（n=15）.The primary HCC precancerous lesion model was established by intraperitoneal injection of DEN（50mg/kg）,once every 3days for 10 weeks.Moxibustion was applied to bilateral＂Ganshu＂（BL 18）for about 15min（3 moxacones）,or 30min（six moxa-cones）,with or without ginger-slice separation,once every other day for 10 consecutive weeks.The contents of T cells of CD3^＋,CD4^＋,CD8^＋and CD4^＋/CD8^＋ ratio in the peripheral blood were detected with Flow Cytometey（FCM）,pathological changes of liver were observed by light microscope after hematoxylin-eosin（HE）stain.ResultsCompared to the control group,the contents of blood CD3^＋ and CD4^＋ T cells and ratio of CD4^＋/CD8^＋ were significantly down-regulated,while that of CD8^＋T cells was obviously increased in the model group（P〈0.05,P〈0.01）.After moxibustion intervention,the decreased CD3^＋ and CD4^＋ T cells and CD4^＋/CD8^＋ levels and the increased CD8^＋ T cell contents were reversed in all the 3 moxibustion groups（P〈0.05,P〈0.01）,except CD3^＋ in the ginger-separated moxibustion group（P〈0.05）.There were no significant differences among the three moxibustion groups in the CD3^＋,CD4^＋,CD8^＋ and CD4^＋/CD8^＋ levels（P〈0.05）.In addition,the pathological changes of liver tissue as central vein deviation or absence,disordered arrangement of hepatic cords,narrowing of the hepatic sinusoid,hyperplasia of collagen fibers,formation of tuberosis,unevenness of liver cells with nuclear anachromas

关 键 词：肝癌 直接灸 隔姜灸 癌前病变 T淋巴细胞 肿瘤免疫 

分 类 号：R245.8[医药卫生—针灸推拿学]