17α-甲基睾酮在猪毛发中蓄积规律的研究  

Study on the accumulation rule of 17α-methyltestosterone in pig hair by liquid chromatography tandem mass spectrometer

作　　者：李艳芹[1] 赖丽虹 姬竹玲 蒋攀文[1] 方炳虎[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《中国兽医科学》2016年第8期1038-1043,共6页Chinese Veterinary Science

基　　金：广东省自然科学基金项目(S2011020001420)

摘　　要：为建立猪毛发中17α-甲基睾酮残留的液相色谱-串联质谱检测方法,研究多剂量内服、单剂量肌肉注射两种给药方式下,17α-甲基睾酮在猪毛发的蓄积规律及差异,选用体重为（20.0±3.5）kg的杜长大三元杂交猪6头,随机分为2组,一组按体重4 mg/kg内服17α-甲基睾酮、连续给药30 d,另一组按体重4mg/kg单剂量肌肉注射17α-甲基睾酮,毛发样品均采集到给药后第61天。样品处理后经液相色谱-串联质谱仪分析检测。以时间为横坐标、质量分数为纵坐标,分析17α-甲基睾酮在猪毛发中的残留规律。结果显示,17α-甲基睾酮在0.75-1 000 ng/g范围内线性关系良好（r2=0.999 1）;检测限为0.30 ng/g,定量限为0.75 ng/g。多剂量内服给药组,第一次给药后第10天,毛发中药物质量分数达到峰值,而后出现下降,到约16 d后趋于稳定,停药后逐渐下降;单剂量肌肉注射给药组,药物质量分数在给药后第10天达到峰值,而后迅速下降;两种给药途径,在给药后第61天均仍能检测到17α-甲基睾酮。结果表明,建立的检测方法灵敏度较高;多剂量内服给药组在给药30 d及停药后31 d内、单剂量肌肉注射给药组在给药后61 d内均可在猪毛发中检测到17α-甲基睾酮,但不同给药方式之间蓄积差异较大。To develop a liquid chromatography tandem mass spectrometer method for the determination of 17α-methyltestosterone in pig hair,and to observe difference of accumulation of 17α-methyl- testosterone between different approaches,six pigs （20.0 kg-3.5 kg） were randomly assigned to two groups, three of which were fed with a mixture containing feed and 17α-methyltestosterone（4 mg/kg BW per day） for thirty consecutive days, and another three of which were given a single intramuscular injection with 17α-methyltestosterone. The pig hairs were collected over a period of 61 days. The hair samples were conducted and then analyzed by liquid chromatography tandem mass spectrometer system. The accumulation rule was analyzed by time as X-axis and concentration of 17α-methyltestosterone in pig hair as Y-axis. The correlation coefficient was 0.999 1 over a dynamic range of 0.75 to 1 000 ng/g; the limit of detection was 0.30 ng/g, and the limit of quantification was 0.75 ng/g. The peak of concentration appeared on day 10 and following a period of decline in the multiple dose oral 17α-methyltestosterone group, and then a leveling-off was observed on about day 16. The peak of concentration appeared on day 10 and then rapidly declined in the injection 17α-methyltestosterone group. 17α-methyltestos-terone was still detected on day 61 in two groups. In conclusion, a higher sensitive method was obtained. 17α-methyltestosterone was detected in pig hair during the treatment（30 days） and withdraw period （31 days） in oral administration group and 61 days after single intramuscular administration. The signifi- cant diversity of the accumulation rule between different methods of administration was observed.

关 键 词：液相色谱-串联质谱 17α-甲基睾酮 猪毛发 蓄积规律 

分 类 号：S851.4[农业科学—预防兽医学]