橄榄油脂肪乳对脓毒症患者CD4^＋CD8^＋及TNF-αIL-10的影响  

The effects of the olive oil fat emulsion on CD4^＋, CD8^＋, TNF-α and IL-10 of patients with sepsis

作　　者：康秀文 陈晓兵 王言理 张慧利[2] 

机构地区：[1]徐州医科大学附属连云港市医院ICU,江苏连云港222002 [2]南昌大学第一附属医院急诊科,江西南昌330000

出　　处：《中国急救医学》2016年第8期679-681,共3页Chinese Journal of Critical Care Medicine

基　　金：连云港市科技局社会发展计划项目（SH1001）

摘　　要：目的探讨橄榄油脂肪乳在脓毒症患者中的应用价值。方法选择我院ICU脓毒症患者31例，随机分为两组，A组15例：中长链脂肪乳（力能）干预组；B组16例：橄榄油脂肪乳（克林诺）干预组。两组患者均行积极抗感染等基础治疗，A组给予中长链脂肪乳，B组给予橄榄油脂肪乳共5d。两组患者于治疗前和治疗后第6天清晨抽取外周静脉血进行指标检测，流式细胞仪检测CD4^＋、CD8^＋的表达，双抗体夹心酶联免疫吸附法（ELISA）检测TNF-α、IL-10浓度。结果两组患者治疗前CD4^＋、CD8^＋、CD4^＋／CD8^＋和TNF—α、IL-10差异无统计学意义（P〉0．05）；治疗后橄榄油脂肪乳干预组较中长链脂肪乳干预组CD4^＋比例降低，CD8^＋比例升高，CD4^＋／CD8^＋比值降低，TNF—α浓度降低，IL-10浓度升高，差异有统计学意义（P〈0．05）。结论橄榄油脂肪乳能降低脓毒症患者TNF—α浓度，提升IL-10浓度，改善CD4^＋／CD8^＋比值。Objective To investigate the value of the olive oil fat emulsion in patients with sepsis. Methods A total of 31 sepsis patients were randomly divided into 2 groups, 15 cases in the medium and long chain fat emulsion group, 16 cases in the olive oil fat emulsion group. The patients were treated actively to fight infection. Two groups of patients were given five days fat emulsion. They were extracted peripheral venous blood to test indicators before treatment and 6 days after treatment. Flow cytometry was used to detect the expression of CD4^＋, CD8^＋, double antibody sandwich enzyme-linked immunosorbent assay was used to detect the concentration of TNF-α and IL-10. Results There was no statistically significant difference between two groups patients before treatment of CD4^＋, CD8^＋, CD4^＋/CD8^＋, TNF-α and IL-10 （P 〉 0.05 ）. Compared with the medium and long chain fat emulsion group, the proportion of CD4^＋ in the olive oil fat emulsion group was reduced, the proportion of CD8^＋ was increased, the ratio of CD4^＋/CD8^＋ was decreased, the concentration of TNF-α was decreased, and the concentration of IL-10 was increased, the differences were statistically significant （P 〈 0.05）. Conclusion In patients with sepsis, the olive oil fat emulsion can reduce the concentration of TNF-α, improve the concen-tration of IL-10 and the ratio of CD4^＋/CD8^＋ ratio.

关 键 词：橄榄油脂肪乳(克林诺) 脓毒症 CD4^+ CD8^+ 

分 类 号：R512.62[医药卫生—内科学]