舒芬太尼后处理对子宫缺血-再灌注大鼠急性肺损伤的影响  

Effect of sufentanil postconditioning on acute lung injury induced by ischemia-reperfusion of uterine in rats

作　　者：李戈辉[1] 黄晓雷[1] 李元涛[1] 齐晓非[1] 孙晶[1] 王晓光[1] 

机构地区：[1]深圳市妇幼保健院麻醉科,518000

出　　处：《临床麻醉学杂志》2016年第8期791-793,共3页Journal of Clinical Anesthesiology

基　　金：2014年度深圳市卫生计生系统科研项目(201402060)

摘　　要：目的研究舒芬太尼后处理对子宫缺血-再灌注(IR)大鼠急性肺损伤的影响。方法成年雌性SD大鼠45只,随机分为三组:对照组(C组)、IR组和舒芬太尼后处理组(SPC组),每组15只。IR组、SPC组建立子宫缺血45 min再灌注2 h模型,SPC组给予舒芬太尼后处理。检测大鼠处理后子宫、血清及肺组织中TNF-α、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性以及肺湿干重比(W/D)、肺通透性指数(LPI)。结果 IR组、SPC组子宫、血浆及肺脏中TNF-α、MDA含量、W/D、LPI明显高于C组(P<0.05);SPC组TNF-α、MDA含量、W/D、LPI明显低于IR组(P<0.05);IR组、SPC组子宫、血浆以及肺脏中SOD活性明显低于C组(P<0.05);SPC组SOD活性明显高于IR组(P<0.05)。结论舒芬太尼后处理能减轻子宫缺血-再灌注大鼠急性肺损伤,可能与减少氧自由基和炎症因子的产生,降低肺通透性,减轻肺水肿,从而减轻子宫缺血-再灌注大鼠引发的急性肺损伤有关。Objective To investigate the effect of sufentanil postconditioning on acute lung injury induced by ischemia-reperfusion of uterine in rats. Methods Fourty-five adult female Sprague-Dawley rats were randomly divided into 3 groups（ n = 15 each） ： control group（ group C）,ischemia-reperfusion group（ group IR） and sufentanil postconditioning group（ group SPC）. Group SPC received sufentanil 10 μg/kg via intraperitoneal injection before inducing reperfusion of uterine. Ischemia-reperfusion of uterine was produced by occlusion of bilateral uterine arteries for 45 min followed by reperfusion for 2 h in group IR and group SPC. Then the content of tumor necrosis factor-α（ TNF-α）,malodiadehyde（ MDA） and superoxide dismutase（ SOD） activity were measured in uterus,serum and lung tissue,lung wet to dry weight ratio（ W /D）,lung permeability index（ LPI） were compared. Results Compared with group C,TNF-α,MDA content,W / D and LPI in uterus,serum and lung tissue were significantly increased in group IR and group SPC（ P〈0. 05）. TNF-α,MDA content,W/D and LPI in uterus,serum and lung tissue were significantly attenuated in group SPC as compared with group IR（ P〈0. 05）. Compared with group C,SOD activity in uterus,serum and lung tissue was significantly attenuated in group IR and group SPC（ P〈0. 05）. SOD in uterus,serum and lung tissue were significantly increased in group SPC as compared with group IR（ P〈0. 05）. Conclusion Sufentanil postconditioning attenuates pulmonary injury caused by ischemia-reperfusion of uterine in rats by inhibiting the inflammatory reaction and suppressing the activation of oxyradical.

关 键 词：舒芬太尼 子宫缺血-再灌注 急性肺损伤 氧自由基 炎性因子 

分 类 号：R614[医药卫生—麻醉学]