胎儿宫内发育迟缓孕妇胎盘组织中差异表达基因及相关信号通路分析  

Analysis of differentially expressed genes and related signaling pathways in placental tissues of pregnant women with intrauterine growth restriction

作　　者：陆元庆[1] 吕明明[2] 

机构地区：[1]南京医科大学附属南京妇幼保健院产科,江苏省210004 [2]南京医科大学附属南京妇幼保健院医学研究中心,江苏省210004

出　　处：《江苏医药》2016年第16期1768-1771,共4页Jiangsu Medical Journal

基　　金：南京医科大学医学科技发展基金重点项目(2014NJMUZD048)

摘　　要：目的筛选胎儿宫内发育迟缓(IUGR)孕妇胎盘中异常表达的基因和可能参与该疾病的信号通路。方法从GEO数据库中下载GSE35574芯片数据,包含35例IUGR孕妇胎盘和40例正常孕妇胎盘组织样本的表达谱数据。选取两组间P值<0.05的差异基因,进行GO功能注释和信号通路分析,揭示IUGR相关的信号通路。结果芯片数据分析显示,在IUGR的胎盘中,差异倍数1.2以上(P<0.05)的基因有108条;其中,表达上调的基因61条,表达下调的基因47条。对IUGR胎盘组织中差异基因进行基因功能注释。GO功能注释分析结果显示,上调基因相关的基因功能注释有232条,下调基因相关的基因功能注释有151条。信号通路分析结果显示,上调基因相关的有显著差异的信号通路有43条,下调基因相关的有显著差异的信号通路有34条。GO功能注释和信号通路分析的结果共同表明IUGR有可能与代谢、免疫事件有关。结论本研究比较系统地揭示了IUGR胎盘中差异基因的表达谱特征和所涉及的重要信号通路。代谢、免疫事件相关的差异基因可能与IUGR有关。Objective To screen the differentially expressed genes and identify potential signaling pathway in placental tissues of pregnant women with intrauterine growth restriction. Methods The gene microarray dataset of placental tissues of intrauterine growth restriction, GSE35574 was downloaded from GEO for screening the differentially expressed genes in 35 cases with intrauterine growth restriction（group A） and 40 normal pregnant women（group B）. The differentially expressed genes with P〈0. 05 were obtained in two groups. The GO and pathway analysises were used to reveal the signaling pathways related to the intrauterine growth retardation. Results Analysis of the microarray data identified 108 differentially expressed genes in placenta tissues in group A（fold change〉1. 2,P〈0. 05）, of which 61 genes were up-regulated and 47 genes were down-regulated. CA） analysis showed that up-regulated genes with significant difference gene function annotation were 232 and down-regulated genes were 151. Significant difference pathways related to up-regulated genes were 43 and related to down-regulated genes pathway were 34. The results of GO and pathway analysis showed that intrauterine growth retardation could be related to metabolism and immune events. Conclusion The study shows differential gene expression profile and potential signaling pathway in placental tissues of pregnant women with intrauterine growth restriction. The differential genes related to metabolism and immune events may be closely related to the intrauterine growth restriction.

关 键 词：胎儿宫内发育迟缓 胎盘 差异基因 芯片 信号通路 

分 类 号：R714[医药卫生—妇产科学]