消癌平注射液提取浓缩工艺的优化  

Optimization of extraction and concentration process for Xiaoaiping Injection

作　　者：李存玉[1,2] 马赟[1] 李贺敏[1] 陈涛[1] 李红阳[1] 彭国平[1,2] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中成药》2016年第8期1712-1715,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81503258;81373980);江苏省自然科学基金青年基金(BK20151005);江苏省中医药局科技项目(YB2015009)

摘　　要：目的采用充氮-纳滤耦合技术优化消癌平注射液的提取浓缩工艺。方法以绿原酸及总酚酸为指标,采用HPLC和紫外可见分光光度法分析两者含有量的变化,比较常规方法与充氮-纳滤耦合技术的提取浓缩效果。结果充氮提取后,绿原酸及总酚酸的含有量分别提高了33.68%和22.22%;纳滤浓缩后,两者分别提高了174.73%和97.42%。结论该技术重复性好,可应用于中药制剂生产过程中的提取和浓缩,尤其是对含有易氧化成分者。AIM To optimize the extraction and concentration process of Xiaoaiping Injection （ an anti-tumor agent） by nitrogen pressurization-nanofiltration coupling technology. METHODS With chlorogenic acid and to- tal phenolic acids as indices, their content changes were determined by HPLC and uhraviolet-visible spectrophotom- etry. The performances of conventional method and nitrogen pressurization-nanofiltration coupling technology in ex- traction and concentration were compared. RESULTS The contents of chlorogenic acid and total phenolic acids were increased by 33.68% and 22.22% due to the application of pressurized nitrogen, and the increasement reached at 174.73% and 97.42% respectively after the successive nanofihration concentration. CONCLUSION With a good reproducibility, nitrogen pressurization-nanofiltration coupling technology can be used for the extrac- tion and concentration of Chinese herbal preparations, especially for those containing easily-oxidized constituents.

关 键 词：消癌平注射液 绿原酸 总酚酸 提取浓缩工艺 充氮-纳滤耦合技术 HPLC 紫外可见分光光度 

分 类 号：R284.2[医药卫生—中药学]