HPLC-DAD法同时测定右归丸中6种成分  

Simultaneous determination of six constituents in Yougui Pills by HPLC-DAD

作　　者：邢婧[1] 张琳[1] 汪轩 章建华[2] 尹华[1] 

机构地区：[1]浙江中医药大学药学院中药标准化研究实验室,浙江杭州310053 [2]浙江中医药大学附属第一医院,浙江杭州310006

出　　处：《中成药》2016年第8期1736-1740,共5页Chinese Traditional Patent Medicine

基　　金：2012年国家自然科学基金项目(81273772);2016年度浙江省新苗人才计划创新孵化项目(2015R410014);浙江省中药学学科科研开放基金资助(Yao2016005);浙江省中医药科技计划项目(2010ZA026)

摘　　要：目的建立高效液相色谱-二极管阵列检测器（HPLC-DAD）法同时测定右归丸（熟地黄、附子、肉桂等）中6种成分的含有量。方法分析采用Waters X Bridge-C18色谱柱（4.6 mm×250 mm,5μm）;流动相甲醇-1%冰醋酸,梯度洗脱;柱温30℃;体积流量1.0 m L/min。结果绿原酸、莫诺苷、松脂醇二葡萄糖苷、马钱苷、阿魏酸和肉桂酸均呈良好的线性关系（r≥0.999）,平均加样回收率（n=6）为99.7%~102.2%（RSD〈3%）。结论该方法简便快速,准确可靠,重复性好,可用于右归丸的质量控制。AIM To establish a high-performance liquid chromatography with diode array detection （HPLC- DAD） method for simultaneously determining the contents of six constituents in Yougui Pills （an osteo joint ease containing Rehmanniae Radix Praeparata, Aconiti Lateralis Radix Praeparata, Cinnamomi Cortex, etc. ）. METH- ODS The analysis was performed on a 30 ℃thermostatic Waters X Bridge-Cls column （4.6 mm × 250 mm, 5 尹华hm） , with the mobile phase comprising of methanol-l% glacial acetic acid flowing at 1.0 mL/min. RESULTS Chlorogenic acid, morroniside, pinoresinol diglucoside, loganin, ferulic acid and cinnamic acid displayed good linear relationships （ r ≥ 0. 999） , whose average recoveries （ n = 6） were 99.7% - 102.2% （ RSD 〈 3% ）. CONCLUSION With good reproducibility, this simple and accurate method can be used for the rapid quality control of Yougni Pills.

关 键 词：右归丸 绿原酸 莫诺苷 松脂醇二葡萄糖苷 马钱苷 阿魏酸 肉桂酸 HPLC-DAD 

分 类 号：R927.2[医药卫生—药学]