清肠栓质量标准研究  

Quality standard for Qingchang Suppository

作　　者：韩柱[1] 崔波[1] 陆菁[1] 安叡[1] 许丽雯[2] 王新宏[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海中医药大学附属龙华医院,上海200032

出　　处：《中成药》2016年第8期1749-1753,共5页Chinese Traditional Patent Medicine

基　　金：上海市卫生局中药新药及院内制剂研发项目(2011XY007)

摘　　要：目的建立清肠栓（三七、青黛、五倍子等）的质量标准。方法 TLC法对三七、青黛、五倍子和马齿苋进行定性鉴别,HPLC法定量测定三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含有量。结果 TLC斑点清晰,分离度好。三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1分别在1.007 5~8.06μg（r=0.999 9）、0.99~7.92μg（r=0.999 1）、1.012 5~8.1μg（r=0.999 1）范围内线性关系良好,平均回收率均大于95%。结论该方法可靠准确,专属性好,可用于清肠栓的质量控制。AIM To establish the quality standard for Qingchang Suppository （a medication for ulcerative coli- tis, made from Notoginseng Radix et Rhizoma, Indigo Naturalis, Rhois Chinensis Calla , etc. ）. METHODS TLC was adopted in the qualitative identification of Notoginseng Radix et Rhizoma, Indigo Naturalis, Rhois Chinen- sis Galla and Portulacae Herba. HPLC was applied to quantitatively determining the contents of notoginsenoside Ra , ginsenosides Rgl and ginsenosides Rb1. RESULTS The TLC spots were clear and well-separated. Notoginsen- oside R1 , ginsenosides Rg1 and ginsenosides Rb1 showed good linear relationships within the ranges of 1. 007 5 - 8.06 μg （ r = 0. 999 9 ）, 0.99 - 7.92 μg （ r = 0. 999 1 ） and 1. 012 5 - 8. 1 μg （ r = 0. 999 1 ） , respectively, with all the average recoveries of more than 95% . CONCLUSION With good specificity, this reliable and accu- rate method can be used for the quality control of Qingchang Suppository.

关 键 词：清肠栓 三七 青黛 五倍子 马齿苋 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 

分 类 号：R927.2[医药卫生—药学]