千佛菌对T1DM模型小鼠Th1∕Th2类细胞因子基因异常表达的双向调节作用  

Effect of Grifola Frondosa on Mice with Type 1 Diabetic Mellitus by Regulating the m RNA Expression of Th1/Th2 Cytokines

作　　者：胡文波[1] 席孝贤[2] 贺新怀 

机构地区：[1]渭南职业技术学院,陕西渭南714026 [2]陕西中医药大学,陕西咸阳712046

出　　处：《四川中医》2016年第8期47-49,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：渭南职业技术学院青年科研基金项目--千佛菌对Th1∕Th2类细胞因子基因表达的调节作用(编号:WZYQ201401)

摘　　要：目的:研究千佛菌对1型糖尿病模型小鼠细胞因子基因异常表达的调节作用及血糖水平、小鼠行为变化的影响,以探讨千佛菌对1型糖尿病小鼠模型的双向免疫调节作用。方法:观察千佛菌大、小剂量组、模型对照组与正常对照组小鼠行为变化;血糖的水平;淋巴细胞IL-2m RNA、IL-10m RNA的表达水平。结果:模型对照组小鼠表现为"三多一少"的症状:饮食多,饮水多,尿量增多,体型瘦弱。千佛菌大、小剂量组症状较模型对照组有所减轻,而正常对照组未见异常。与正常对照组比较,模型对照组血糖水平、IL-2m RNA表达水平明显增高(P<0.01),而IL-10m RNA表达水平明显降低(P<0.01);与模型对照组比较,千佛菌大、小剂量组的血糖水平、IL-2m RNA表达水平均明显降低(P<0.01、P<0.05),IL-10m RNA表达水平均明显增高(P<0.01、P<0.05);而千佛菌大、小剂量组的血糖水平、IL-2m RNA及IL-10m RNA表达水平的差异无统计学意义(P>0.05)。结论:千佛菌具有降低1型糖尿病模型小鼠血糖的作用,能下调IL-2m RNA的表达,上调IL-10m RNA的表达,对1型糖尿病模型小鼠脾脏淋巴细胞Thl/Th2细胞因子的平衡具有调节作用。Objective： In order to investigate the two- way adjustment of Grifola frondosa against mice with type 1diabetic mellitus（ T1DM）. Methods： The behavior of all mice was observed. The glucose level in blood was tested by Blood Glucose Meter. The RNA was extracted in the spleen and the IL-2m RNA and IL-10 m RNA expression of Th1 / Th2 cytokines were detected by Real-time PCR. Results： The model of T1 DM showed the symptoms of polydipsia,polyuria,poly-phagia and weight loss. The symptoms of the mice in the therapy group were alleviated. Compared with the controlled group,the glucose level in blood and the expression of IL-2m RNA all increased obviously（ P0. 01）,but the expression of IL-10 m RNA decreased significantly in model group（ P0. 01）; compared with the model group,the glucose level in blood and the expression of IL- 2m RNA all decreased significantly（ P0. 01,P0. 05）,but the expression of IL-10 m RNA all increased obviously in therapy group 1and group 2（ P0. 01,P0. 05）. However,there was no significant difference in the glucose level in blood and the expression of IL- 2m RNA and IL-10 m RNA between the therapy group 1and group 2（ P0. 05）. Conclusion： Grifola frondosa is beneficial to lower the glucose level in blood in mice with T1 DM. At the same time,it can also decrease significantly the expression of IL-2m RNA and enhance the expression of IL-10 m RNA. Grifola frondosa plays a role in regulating the balance of Th1 / Th2 cytokines by the two-way adjustment.

关 键 词：1型糖尿病 千佛菌 IL-2MRNA IL-10mRNA 荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]