疣蝗转录组分析  

De novo Assembly and Analysis of the Trilophidia annulata Transcriptome

作　　者：邱忠营[1] 刘菲[1,2] 张克瑶 黄原[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]陕西学前师范学院生命科学与食品工程学院,西安710061

出　　处：《基因组学与应用生物学》2016年第8期1989-1998,共10页Genomics and Applied Biology

基　　金：国家自然科学基金(31372192)资助

摘　　要：疣蝗分布广泛,是一种常见的害虫,主要危害农作物,但分子信息缺乏。我们采用高通量Illumina测序技术和从头组装的方法获得了疣蝗转录组unigenes,并鉴定了天然免疫相关信号通路基因。本研究共获得疣蝗转录组unigenes 86 756个,平均长度为670 bp,N50为1 033 bp。使用六大数据库Nr、Nt、Swiss-Prot、GO、COG和KEGG注释疣蝗转录组unigenes,分别对应有29 627,13 866,23 398,13 474,11 968和21 027个unigenes获得注释。本研究共鉴定319个天然免疫信号通路相关的unigenes,其中,参与Toll通路的unigenes 49个,参与免疫缺失(IMD)通路的unigenes 13个,参与JAK-STAT信号通路的unigenes 117个,参与MAPK-fly通路相关的unigenes 58个,参与黑化(Melanization)通路的unigenes 82个。另外,我们还预测到了9 287个微卫星(SSRs)。本研究为疣蝗基因功能分析、免疫通路相关基因和分子标记的进一步研究提供了分子理论基础。Trilophidia annulata is widely distributed as a common pest, mainly damaging crops. However, there is a lack of the molecular information. We used high-throughput Illumina sequencing technology to obtain T. annulata transcriptome data using de novo assembly approach. Moreover, we identified the innate immune signaling pathways related genes. In this study, we obtained 86 756 unigenes of T. annulata with an average length of 670 bp and N50 of 1 033 bp. Using six databases （Nr, Nt, Swiss-Prot, GO, COG and KEGG databases）, we got 29 627, 13 866, 23 398, 13 474, 11 968 and 21 027 unigenes, respectively. This study identified 319 innate immune signaling pathways associated unigenes. 49 unigenes are involved in Toll pathway; 13 unigenes are invol- ved in immunodeficiency （IMD） pathway; 117 unigenes are involved in JAK-STAT signaling pathway; 58 unigenes are involved in MAPK-fly pathway; and 82 unigenes are involved in melanization pathway. In addition, we also identified 9 287 SSRs. T. annulata transcriptome data provided a molecular basis for the gene function analysis, innate immune pathway investigation and molecular marker discovery.

关 键 词：疣蝗 转录组 天然免疫 SSRS 

分 类 号：S433[农业科学—农业昆虫与害虫防治]