基于28S rRNA基因序列的中国寄蝇亚科部分种类分子系统发育研究(双翅目:寄蝇科)  

Molecular Phylogenetic Analysis of Some Tachininae Species (Diptera: Tachinidae) from China Based on 28S rRNA Gene Sequences

作　　者：智妍[1,2] 李新[1] 刘家宇[2] 葛振萍[3] 赵喆[1,4] 张春田[1] 

机构地区：[1]沈阳师范大学生命科学学院,沈阳110034 [2]贵州医科大学,贵阳110025 [3]沈阳职业技术学院,沈阳110045 [4]中国科学院动物研究所,北京100083

出　　处：《基因组学与应用生物学》2016年第8期1999-2006,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(31272279;31093430);贵州省科学技术基金(LH[2015]7353;J[2013]2055)共同资助

摘　　要：本研究基于中国地区双翅目寄蝇科寄蝇亚科5族10属17种昆虫的28S r RNA基因序列,分别利用PAUP4.0b和MEGA5.05软件的邻接法(NJ)、最大简约法(MP)和最小进化法(ME),选取追寄蝇亚科的黄足突额寄蝇Biomeigenia flava和麻蝇科的红尾拉麻蝇Ravinia striata为外群,重建寄蝇亚科部分种类的系统发育树。结果表明:测得的696 bp寄蝇亚科核苷酸片段,包括127个变异位点和52个简约信息位点;不同方法得到的系统发育树的拓扑结构基本一致。本研究结果部分解决了基于形态学定义的该亚科内族间、属间的系统发育关系:(1)支持寄蝇亚科、寄蝇族分别为一个单系群;(2)支持形态分类的将诺寄蝇属Nowickia Wachtl并入寄蝇属Tachina Meigen并作为后者的一个亚属的结论;(3)同时支持将短须寄蝇族Linnaemyini并入埃内寄蝇族Ernestiini。In this study, we used Neighbor-joining （N J）, Maximum-parsimony （MP） and Minimum-evolution （ME） methods of PAUP4.0b and MEGAS.05 softwares, respectively selecting Biome ige nia flay a in subfamily Exoristinae and Ravinia striata in family Sarcophagidae as out-groups, re-establishing different kinds ofphylogenetic trees for Tachininae. This research was based on 28S rRNA gene sequences of 17 species （Diptera： Tachinidae： Tachininae） which were in 10 genera, 5 tribes in China. The results showed that the 696 bp nucleotide fragments of the subfamily included 127 mutation sites and 52 simple information sites, and the topological structures of phylog- enetic trees which were gotten by adopting various methods were basically coincide. The results partially solved the relationships of main tribes and genera of Tachininae defined by morphology, proved that Tachininae and Tachinini could respectively belong to one monophyletic group, supported that Nowickia Wachtl should be fallen under Tachina Meigen as its subgenus, and also provided evidence on a molecular level for combining Linnae- myini and Ernestiini into one tribe.

关 键 词：寄蝇亚科 双翅目 28S RRNA基因 系统发育 中国 

分 类 号：Q969[生物学—昆虫学]