cDNA-AFLP技术在植物生物胁迫和非生物胁迫中的应用  

The Applications of cDNA-AFLP Technology in Plant Biotic Stress and Abiotic Stress

作　　者：刘冠[1] 赵婷婷[1] 杨欢欢[1] 张冬野[1] 姜景彬[1] 李景富[1] 许向阳[1] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030

出　　处：《基因组学与应用生物学》2016年第8期2186-2193,共8页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31272171);黑龙江省杰出青年科学基金项目(JC201204);现代农业体系技术专项资金项目(CARS-25-A-15)共同资助

摘　　要：c DNA-AFLP技术因其具有多态性丰富、且效率较高,而且稳定性强等优势,被广泛的应用在植物差异表达的各个领域。在植物的生物胁迫和非生物胁迫方面,该技术不断完善,并且取得了很多研究成果。本综述从c DNA-AFLP技术的发展建立、原理、技术特点和在植物生物胁迫和非生物胁迫中的应用等方面进行简单阐述,并对该技术进行了展望。本综述重点讨论了该技术生物胁迫中的两方面即抗病育种和抗虫育种中的应用,以及在植物非生物胁迫中耐热、耐冷、耐盐、耐旱等方面的应用,突出显现了c DNA-AFLP在植物应用中至关重要的地位,同时还简要列举了一些应用实例。cDNA-AFLP technology has been widely applied in various fields of plant differentiated expressions because of the advantages such as rich polymorphism, high efficiency and strong stability. In the aspects of biotic stress and abiotic stress, this technology is constantly improved and it has achieved many research achievements. This review discussed the foundation and development, principle, technical characteristics, and application in plant biotic stress and abiotic stress of cDNA-AFLP technology, and predicted the prospect of this technology. This review focused on the discussions about the application of this technology in biotic stress, such as disease resistance breeding and insect resistance breeding, and the application of this technology in abiotic stress, such as heat resistance, cold resistance, salt resistance and drought resistance. This review highlighted the importance of cDNA-AFLP in plant applications, and briefly enumerated some application examples.

关 键 词：CDNA-AFLP 生物胁迫 非生物胁迫 

分 类 号：Q943.2[生物学—植物学]