应用慢性HBV感染者家系探索HBV基因变异率差异的影响因素  

Influential factors of HBV gene mutation rate among mother-child patients with chronic HBV infection

作　　者：施毅[1] 张振华[1] 孙秋林[1] 魏少峰[1] 苏倩[1] 张亚飞[1] 李旭[1] 

机构地区：[1]安徽医科大学第一附属医院感染病科,安徽合肥230022

出　　处：《中华疾病控制杂志》2016年第8期835-838,共4页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(81273142)

摘　　要：目的探讨不同患者乙型肝炎病毒（hepatitis B virus,HBV）基因变异率情况及影响因素。方法选取4组慢性HBV感染者家系共6对母子为研究对象,PCR扩增HBV全长序列,分析其基因变异率并探讨其与疾病进程的相关性。结果 6对母子均为B基因型HBV感染,不同患者间HBV核苷酸变异率分别为6.67×10^-5、2.83×10^-5、2.33×10^-5、1.48×10^-4、1.83×10^-4、1.24×10^-4个变异/位点/年,差异可高达7.85倍,其中家系内HBV变异率差异分别为2.36和6.35倍。免疫耐受期HBV基因变异率2.83×10^-5和1.24×10^-4个变异/位点/年,非免疫耐受期HBV基因变异率2.33×10^-5~1.83×10^-4个变异/位点/年。各编码区变异率亦有所差异,但差异均无统计学意义（x^2核苷酸=2.812,P=0.422;x^2氨基酸=2.344,P=0.504）。结论不同患者HBV基因变异率有较大差异,且可能与患者所处HBV感染自然病程有关。Objective To study the nucleotide substitution rate of full-length hepatitis B virus（ HBV） in patients who were infected with HBV and its influential factors. Methods Six pairs of mother-child patients with chronic HBV infection were included as the study subjects. Full-length HBV was amplified by PCR from sera samples and the nucleotide substitution rate their correlation with disease processes were analyzed. Results Six pairs of mother-child patients were all HBV B genotype. The nucleotide substitution rates were 6. 67 × 10^-5,2. 83 × 10^-5,2. 33 × 10^-5,1. 48 × 10^-4,1. 83 ×10^-4,1. 24 × 10^-4 substitution / site / year respectively and the differences was up to 7. 85 times,in which the HBV nucleotide substitution rates within pedigrees were 2. 36 and 6. 35 times. The HBV nucleotide substitution rates during immune tolerance phase were 2. 83 × 10^-5and 1. 24 × 10^-4substitution / site / year,while the rates during the non-immune tolerance phase were 2. 33 × 10^-5to 1. 83 × 10^-4substitution / site / year. The nucleotide substitution rate of every coding region was different,but there was no significant difference（ nucleotide： x^2= 2. 812,P = 0. 422; amino acids： x^2= 2. 344,P =0. 504）. Conclusions The nucleotide substitution rates of full-length HBV in different patients are quite different which may be associated with the natural course of HBV infection.

关 键 词：肝炎病毒 乙型 碱基序列 流行病学方法 

分 类 号：R512.62[医药卫生—内科学] R181.2[医药卫生—临床医学]