昆仑雪菊活性成分的高效液相色谱测定方法改良及不同品系成分的测定  

A modified HPLC method for detection of main active ingredients in different strains of Kunlun Chrysanthemum

作　　者：阮飞[1] 张宽朝[1] 何孔泉[1] 金青[1] 王亚哲[1] 蔡永萍[1] 林毅[1] 宋程[1] 杨家平[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《安徽农业大学学报》2016年第4期646-650,共5页Journal of Anhui Agricultural University

基　　金：安徽省科技攻关项目(11011402197;12011402136)资助

摘　　要：采用改良的高效液相色谱方法,以贡菊为对照,测定了不同品系昆仑雪菊中绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸及槲皮苷的含量。结果表明,较适宜的高效液相色谱条件为Alitima HP C18 5μm色谱柱(250mm×4.6 mm),以乙腈-0.1%磷酸水溶液梯度洗脱,流速1 m L·min-1,检测波长为350 nm,柱温40℃,进样量5μL。不同品系的昆仑雪菊与贡菊主要活性成分相似,但其含量差异显著。用该方法测得昆仑雪菊和贡菊中的绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸及槲皮苷的含量均高于药典规定的含量标准,其中昆仑雪菊中绿原酸、木犀草苷和槲皮苷的含量显著高于供试贡菊。The contents of chlorogenic acid, galuteolin, 3, 5-O-caffeoylquinic acid, and quercitrin in different strains of Kunlun Chrysanthemum （Coreopsis tinctoria） were determined using a modified high performance liquid chromatography （HPLC） method with Florists Chrysanthemum as the control. The results showed that the suitable HPLC conditions were： Alitima HP C18 5 μm chromatographic column （250 mm×4.6 mm）, acetonitril-0.1%phosphoric acid aqueous gradient elution, flow rate of 1 mL.min-1, detection wavelength of 350 nm, column temperature of 40℃, and sampling volume of 5 μL. The main active ingredients in different strains were similar with those in Florists Chrysanthemum, but their contents were significantly different. The contents of four substances measured by the modified method were higher than those in the Chinese Pharmacopoeia＇s standards. The contents of chlorogenic acid, galuteolin and quereitrin in Kunlun Chrysanthemum were significantly higher than those in Florists Chrysanthemum.

关 键 词：昆仑雪菊 贡菊 活性成分 高效液相色谱 

分 类 号：S567.2[农业科学—中草药栽培]