MiRNA376a/c靶向调控TGFA对CD133^+ U251胶质瘤细胞增殖的影响  

Effects of Mi RNA376a/c target regulation of TGFA on CD133^+ U251 glioma cell proliferation

作　　者：杜鹏[1] 栾新平[1] 木依提[1] 苑杨[1] 徐敬轩[1] 张晶晶[1] 杨岩[1] 

机构地区：[1]新疆医科大学第二附属医院神经外科,乌鲁木齐830063

出　　处：《重庆医科大学学报》2016年第8期827-832,共6页Journal of Chongqing Medical University

基　　金：新疆自治区基金资助项目(编号:2015211C108);阿勒泰地区基金资助项目(编号:201510)

摘　　要：目的:探讨微小核糖核酸(Micro RNA,Mi RNA)376a/c靶向调控转化生长因子α(transforming growth factor alpha,TGFA)在CD133+U251胶质瘤细胞增殖中作用方式及其机制。方法:构建Mi R376a/c靶向调控TGFA慢病毒克隆体,根据Mi R376a/c与TGFA结合位点分为4组,检测各组酶活性。根据转染体外Mi R376a/c和Mi R376a/c模拟物(Mi R-SCR)不同,将CD133+U251细胞分为4组,通过细胞培养、Gimsa染色,检测TGFA蛋白表达量和细胞生长曲线,观察细胞增殖情况,收集资料并进行统计学分析。结果:CD133+组与CD133-组Mi R376a/c表达比较,差异有统计学意义(P<0.001);wt-TGFA组与mut-TGFA组质粒荧光素酶活性表达差异比较,差异有统计学意义(P=0.000);将Mi R376a/c慢病毒导入CD133+U251细胞中,Mi R376a/c组TGFA蛋白表达相对于Mi R-SCR组和空白对照组明显下调,差异有统计学意义(P=0.000);生长曲线检测示:随时间延长,Mi R376a/c组细胞生长速度慢于对照组,差异有统计学意义(P=0.000)。结论:Mi RNA376a/c靶向调控TGFA抑制CD133+U251胶质瘤细胞增殖、克隆。Objective：To investigate the mode and mechanism of Mi RNA376a/c targeted regulation of transforming growth factor alpha（TGFA）in CD133＋ U251 glioma cell proliferation. Methods：Lentivirus clones of Mi R376a/c target regulation of TGFA was built and were divided into four groups according to Mi R376a/c and TGFA binding sites. Enzyme activity of each group was detected. CD133＋U251 cells were divided into four groups according to the transfection in Mi R376a/c and Mi R-SCR. Cell culture and Gimsa staining were used to detect and observe TGFA protein expression,cell growth curve,and cell proliferation. Information was collected and sta tistical analysis was conducted. Results ：There were statistically significant differences in Mi R376 a / c expression between CD133 ＋group and CD133-group（P=0.000）. There were statistically significant differences in plasmid luciferase activity expression between wt-TGFA group and mut-TGFA group（P=0.000）. Mi R376a/c lentivirus was imported into CD133＋ U251 cells and TGFA protein ex pression was down regulated in Mi R376a/c group than in Mi R-SCR group and blank control group,with statistical differences（P〈0.001）. Growth curve test showed that with the extension of time,cell growth speed was slower in Mi R376 a group than in control group,with statistical significant differences（P=0.000）. Conclusion：Mi RNA376a/c target regulation of TGFA inhibits clone and proliferation of CD133＋U251 glioma cells.

关 键 词：MI RNA376a/c CD133+U251 增殖 靶向调控 

分 类 号：R64[医药卫生—外科学]