泡核桃干腐病病原菌分离鉴定及杀菌剂室内筛选  

Identification of the Pathogen Causing Canker on Juglans sigilltata L.and Indoor Screening of Fungicides

作　　者：陈丽华[1] 张家恒[1] 吴毅歆[2,3] 何鹏飞[1,3] 刘凌[4] 闫争亮[4] 何月秋[2,3] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]微生物菌种筛选与应用国家地方联合工程研究中心,云南昆明650217 [4]云南省林业科学院,云南昆明650204

出　　处：《云南农业大学学报（自然科学版）》2016年第4期624-629,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家科技支撑计划(2011BAD46B01-3)

摘　　要：为明确泡核桃干腐病的病原和筛选合适的化学防治药剂,从云南省楚雄州大姚县核桃病害标本中分离和鉴定了病原菌,测定了8种杀菌剂的毒力。通过柯赫氏法则验证,确定分离得到的该菌株能侵染泡核桃,产生干腐症状。依据形态特征和r DNA-ITS序列分析,将病原鉴定为茶藨子葡萄座腔菌(Botryosphaeria dothidea)。室内毒力测定的结果表明:8种杀菌剂的EC50从大到小依次为氟啶胺0.060 4 mg/L、戊唑醇0.169 0 mg/L、苯醚甲环唑0.177 8 mg/L、嘧菌酯0.476 9 mg/L、福美双2.094 7 mg/L、噻呋酰胺3.961 0 mg/L、异菌脲4.219 0 mg/L、霜脲氰101.764 3 mg/L。To identify the pathogen causing canker on Juglans sigilltata L. and screen fungicides, the pathogen collected from Dayao County of Yunnan Province was isolated and identified and 8 fungicides were tested in toxicity. By following the Koch＇s postulate, the pathogen was confirmed and was identified as Botryosphaeria dothidea based on its morphology and rDNA-ITS gene sequence. The toxicities in EC50 were 0.0604, 0.1690, 0.1778, 0.4769, 2.0947, 3.961 0, 4.2190 and 101.7643mg/L for fluazinam, tebuconazole, difenoconazole, azoxystrobin, thiram, thifluzamide, iprodione and cymoxanil, respectively.

关 键 词：泡核桃 干腐病 茶藨子葡萄座腔菌 杀菌剂 毒力测定 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]