牛角瓜离体快繁技术研究  

A Study on in vitro Rapid Propagation Technology for Calotropis gigantea

作　　者：唐军荣[1] 李斌[1] 刘惠民[1] 马焕成[1] 郑元[1] 罗明灿[1] 李雪慧[1] 

机构地区：[1]西南林业大学,国家林业局西南地区生物多样性保育重点实验室,云南昆明650224

出　　处：《云南农业大学学报（自然科学版）》2016年第4期658-663,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家林业局林业公益性行业项目(201304810)

摘　　要：以牛角瓜幼嫩顶芽为外植体,研究了0.1%的升汞不同处理时间对无菌顶芽有效成活率的影响,即将所得无菌顶芽去顶后的茎段接种在分别添加了不同质量浓度的6-BA、TDZ、IBA、NAA的MS培养基上,进行离体培养。结果表明:采用0.1%的升汞消毒处理10 min效果最好,牛角瓜顶芽的有效存活率为82.2%;适宜的增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,25 d后的增殖倍数为3.9;组培苗的最佳生根培养基为1/2MS+IBA 0.1 mg/L,其生根率可达100%;将生根苗移栽到混合基质[V(红心土)∶V(腐殖土)∶V(珍珠岩)=2∶2∶1]中,45 d后的移栽成活率为90%以上。牛角瓜快繁体系的成功建立,可为其遗传改良及优良单株的快速繁育提供理论依据及实践指导。The tender apical buds of Calotropis gigantea was used as explants to study the effects of 0.1% HgCl2 at different sterilization times on the livability of the sterile apical buds, and taking the stem segments of aseptic buds as materials, and was cultured on MS culture media with different concentrations of 6-BA, TDZ, NAA, IBA. The result showed that 0.1% HgCl2 sterilization for 10 minutes was the optimal and livability of the sterile apical buds was 82.2%. The rapid propagation medium was MS＋6-BA 1.0mg/L＋NAA 0.1mg/L, where the propagation coefficient was up to 3.9 with 25 days. The optimal medium for rooting was 1/2MS＋IBA 0.1mg/L, and resulted in 100% rooting rate. The survival rate of rooted plantlets was recorded as 90% after 45 days when plantlets were transferred in pots containing mixed substrate[V（red subsoil）：V （humus soil）：V （perlite）=2：2：1）]. The tissue culture and rapid proliferation system was successfully established, and it could provide important theoretical basis and practical guidance for rapid proliferation of superior individuals and its genetic improvement.

关 键 词：牛角瓜 离体快繁 顶芽 

分 类 号：S642.904.3[农业科学—蔬菜学]