利用ISSR和AFLP标记分析石斛种质资源的遗传多样性  

Genetic Diversity among Dendrobium with Known Origins Based on the ISSR and AFLP Markers

作　　者：宋爽[1,2] 周洋帆[1,2] 刘正杰[1,2] 赵明富[2,3] 杨娟[1,2] 徐绍忠[1,2] 文国松[1,2] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学特色资源植物改良与利用研究所,云南昆明650201 [3]云南农业大学植物保护学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2016年第4期688-695,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(81360611);农业科技成果转化资金项目(2013GB2F300441)

摘　　要：本研究利用ISSR和AFLP两种分子标记对51个药用石斛材料(包括35个铁皮石斛种质资源)进行遗传多样性研究。利用ISSR标记筛选出6条引物,共扩增出79条带,平均每条引物扩增条带数约为13.17条,多态条带比率都达到100%。利用AFLP标记筛选出5对引物,共扩增207条带,其中206条具多态性,多态性比率高达99.52%。两标记分析获得的群体种系间关系与变异基本一致,且群体内的分化较小,群体间的分化较大,群体间的基因流动较小。所得到的聚类图表明:ISSR和AFLP标记技术能有效地将研究材料分开,且基本反映了它们之间的亲缘关系,说明两种标记均适合用于石斛植物的遗传多样性研究。The genetic diversity of 51 Dendrobium materials （including 35 D. officinale germplasm resources） was studied by ISSR and AFLP. Six primers chosen from ISSR markers, totally generated 79 amplification bands, on average every primer generate 13.17 amplification bands, and the ratio of polymorphism bands reached 100%. Five primer pairs by AFLP markers, generated altogether 207 bands, 206 of which were polymorphic bands and the average PPB was 99.52%. The results of two molecular markers showed that the level of differentiation between groups was larger, differentiation of groups was comparatively large and gene flow between groups was smaller. The cluster analysis indicated that ISSR and AFLP marker technology could effectively separate the materials, and basically reflect the genetic relationship. Two markers could be applied to detecting genetic diversity analysis of plants in Dendrobium.

关 键 词：石斛 ISSR AFLP 遗传多样性 

分 类 号：R282.71[医药卫生—中药学]