基于UPLC-Q-TOF-MS分析不同产地抱茎苦荬菜化学成分的差异  

Analyze Difference of Chemical Compositions in Ixeris sonchifolia from Different Origins by UPLC-Q-TOF-MS

作　　者：邓皓月[1] 武媛媛[1] 袁磊[1] 王媛[1] 田梦[1] 李遇伯[1] 杨彬[1] 

机构地区：[1]天津中医药大学中药学院,天津300193

出　　处：《中国实验方剂学杂志》2016年第17期37-42,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011ZX09401-305-42)

摘　　要：目的:应用超高效液相色谱-四级杆飞行时间质谱联用技术(UPLC-Q-TOF-MS)分析不同产地抱茎苦荬菜中化学成分差异。方法:采用Waters Acquity BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm),乙腈-0.1%甲酸水为流动相进行梯度洗脱,电喷雾离子源(ESI)正、负离子模式下扫描采集数据,通过准分子离子及二级碎片离子对化学成分进行鉴定。应用主成分分析(PCA)和偏最小二乘判别分析(PLS-DA)对数据进行多元统计分析。结果:从抱茎苦荬菜中鉴定出18种化学成分。其中,11,13α-dihydroixerin Z,木犀草素,3,4-二羟基苯甲酸,芹菜素-7-O-葡萄糖醛酸苷,芦丁,菊苣酸,木犀草素-7-O-葡萄糖醛酸苷及鸟嘌呤核苷为不同产地抱茎苦荬菜的主要差异成分。结论:通过该研究建立的UPLC-Q-TOF-MS多元统计分析方法,明确了不同产地抱茎苦荬菜的差异化学成分,可以为抱茎苦荬菜的质量控制提供依据。Objective： To study the difference of chemical components in Ixeris sonchifolia（ ISH） from different origins by UPLC-Q-TOF-MS. Method： The separation was performed on a Waters Acquity BEH C18column（ 2. 1 mm × 100 mm,1. 7 μm） with acetonitrile-0. 1% formic solution as the mobile phase for gradient elution. Mass spectrometric data were obtained in electrospray ionization（ ESI） positive and negative modes.Meanwhile,the chemical components were identified with quasi-molecular ions and secondary fragment ions.Principal component analysis（ PCA） and partial least squares discriminate analysis（ PLS-DA） were used for multivariate statistical analysis. Result： Eighteen chemical components were identified from ISH. Among them,11,13α-dihydroixerin Z,luteolin,3,4-dihydroxybenzoic acid,apigenin-7-O-glucuronide,rutin,chicory acid,luteolin-7-O-glucuronide and guanosine were the main differential compositions of ISH from different origins.Conclusion： The difference chemical constituents in ISH from different origins were identified by the established UPLC-Q-TOF-MS-MS method,which could provide the basis for the quality control of ISH.

关 键 词：抱茎苦荬菜 不同产地 超高效液相色谱-四级杆飞行时间质谱联用仪 11 13α-dihydroixerin Z 

分 类 号：R284.1[医药卫生—中药学]