白术化学成分分离鉴定  

Chemical Constituents of Atractylodis Macrocephalae Rhizoma

作　　者：邹辉[1] 杨郴 易美玲[1] 吴富旋 盛习锋[1] 冯星[1] 

机构地区：[1]湖南师范大学医学院,长沙410013

出　　处：《中国实验方剂学杂志》2016年第17期43-48,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖南省教育厅资助科研项目(14C0709);湖南省教育厅重点项目(10A071);湖南师范大学博士启动基金项目(130668)

摘　　要：目的:对白术乙酸乙酯提取物的化学成分进行研究。方法:采用多种现代色谱技术进行分离纯化,通过理化性质及光谱方法鉴定化合物的结构。噻唑蓝(MTT)法对所得化合物进行细胞毒活性测试。结果:从白术的乙酸乙酯提取物中分离得到16个化合物,分别鉴定为1-乙酰氧基-6E,12E-二烯-8,10-二炔-3-醇(1),3-乙酰氧基-6E,12E-二烯-8,10-二炔-1-醇(2),6E,12E-十四碳二烯-8,10-二炔-1,3-二醇乙酸酯(3),12-异戊烯酰氧基-14-乙酰氧-2E,8Z,10E-三烯-4,6-二炔-1醇(4),12-异戊烯酰氧基-14-乙酰氧-2E,8E,10E-三烯-4,6-二炔-1醇(5),白术内酯I(6),白术内酯Ⅱ(7),白术内酯Ⅲ(8),双白术内酯(9),杜松脑(10),蒲公英萜醇乙酸酯(11),3β-乙酰氧基-12-齐墩果烯-11酮(12),7-羟基香豆素(13),二十八烷酸(14),十七烷酸(15),7-ɑ-羟基-β-谷甾醇(16)。结论:化合物1,2,16为首次从白术中分离得到,化合物12~15为首次从苍术属植物中分离得到,化合物6对宫颈癌He La细胞系具有较好抑制作用,IC50为15.6μmol·L^(-1)。Objective： To study the chemical constituents of Atractylodis Macrocephalae Rhizoma.Method： The compounds were isolated and purified from ethyl acetate extract of Atractylodis Macrocephalae Rhizoma by using a combination of chromatographic techniques. Their structures were elucidated on the basis of thierphysico-chemical properties and spectroscopic data analyses. MTT method was used to assay the cytotoxicity agianst He La cells. Result： Sixteen compounds were isolated and purified from ethyl acetate extract of Atractylodis Macrocephalae Rhizoma and determined as 1-acetoxytetradeca-6E, 12E-dien-8, 10-diyn-3-ol（ 1）, 3-acetoxytetradeca-6E,12E-dien-8,10-diyn-1-ol（ 2）, tetradeca-6E, 12E-dien-8, 10-diyn-1, 3-diol diacetate（ 3）, 14-acetoxy-12-senecioyloxytetradeca-2E, 8Z, 10E-trien-4, 6-diyn-1-ol（ 4）, 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol（ 5）,atractylenolide Ⅰ（ 6）,atractylenolide Ⅱ（ 7）,atractylenolide Ⅲ（ 8）,biatractylenolide（ 9）,juniper camphor（ 10）,taraxerylacetate（ 11）,3β-acetoxy-12-oleanen-11-one（ 12）,7-hydroxy coumarin（ 13）,octacosanic acid（ 14）,heptadecanoic acid（ 15）,7-α-hydroxyl-β-sitosterol（ 16）. Conclusion： Among them,compounds 1,2,16 were isolated from the species firstly and compounds 12-15 were isolated from the genus of Atractylodes for the first time. Compound 6 showed good inhibitory activity against He La cells with IC50 values of 15. 6 μmol·L-（-1）.

关 键 词：白术 1-乙酰氧基-6E 12E-二烯-8 10-二炔-3-醇 苍术属 细胞毒活性 7-α羟基-β-谷甾醇 

分 类 号：R284.1[医药卫生—中药学]