五味子藤茎提取物抗大鼠肝纤维化作用及机制探讨  

Anti-hepatic Fibrosis Effect and Mechanism of Schisandrae Chinensis Fructus Caulis Extract on Liver Fibrosis in Rats

作　　者：胡彦武[1] 刘凯[2] 闫梦彤 王雷[1] 刘明冬[1] 于俊林[1] 武子敬[1] 

机构地区：[1]通化师范学院制药与食品科学学院,吉林通化134002 [2]吉林大学药学院,长春130021

出　　处：《中国实验方剂学杂志》2016年第17期122-125,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省教育厅"十一五"规划项目(吉教科合字[2008]第216号);通化师范学院科研项目(201271);通化师范学院大学生创新科研项目(201330)

摘　　要：目的:观察五味子藤茎提取物抗四氯化碳(CCl4)及乙醇所致肝纤维化的作用及机制。方法:50只Wistar大鼠随机分为正常组、模型组、五味子藤茎提取物高、中、低剂量组,采用ip CCl4,并饮用乙醇、饲用高脂饲料等复合因素制造肝纤维化模型,造模后,给药组分别ig高、中、低不同剂量(5.0,2.5,1.25 g·kg-1)五味子藤茎提取物,正常组和模型组灌服等量的生理盐水。末次给药后,检测血清丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)活性,层粘蛋白(LN),透明质酸(HA),Ⅲ型前胶原(PCⅢ)水平,同时取大鼠肝组织,分别用于苏木素-伊红(HE)染色观察病理变化,蛋白质免疫印迹(Western blot)法检测转化生长因子-β_1(TGF-β_1),α-平滑肌肌动蛋白(α-SMA)蛋白表达水平。结果:与正常组比较,模型组大鼠血清AST,ALT活性及HA,LN,PCⅢ水平均显著升高,肝组织TGF-β_1,α-SMA蛋白表达水平显著增高(P<0.01);与模型组比较,五味子藤茎提取物能显著降低肝纤维化大鼠血清ALT,AST活性及HA,LN,PCⅢ水平,病理组织学检查结果显示,五味子藤茎提取物各剂量组大鼠肝组织结构明显改善,肝纤维化程度减轻,五味子藤茎提取物各剂量组均可显著降低肝组织TGF-β_1,α-SMA蛋白表达水平(P<0.01)。结论:五味子藤茎提取物对CCl4及乙醇所致肝损伤有明显保护作用,其机制可能与降低肝组织TGF-β_1,α-SMA蛋白表达、抑制肝星状细胞活化有关。Objective： To observe the anti-fibrosis effects and mechanism of Schisandrae Chinensis Fructus caulis extract（ SCFCE） on liver fibrosis in rats caused by CCl4 and alcohol. Method： Totally 50 Wistar rats were randomly divided into normal group,model group,SCFCE high,medium and low dose（ 5. 0,2. 5,1. 25g·kg^-1） groups. Liver fibrosis models were established by intraperitoneal injection of CCl4,drinking alcohol and feeding high fat diets. After modeling,the rats in treatment groups were received different concentrations of SCFCE by intragastric administration,while the rats in the normal group and model group received the same volume of normal saline. After the last administration,the levels of serum alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,laminin（ LN）,hyaluronic acid（ HA）,and procollagen Ⅲ（ PC Ⅲ） were detected,and their hepatic tissues were taken for HE staining. The protein expression levels of transforming growth factor-β1（ TGF-β1）,α-smooth muscle actin（ α-SMA） were detected by Western blot assay. Result： As compared with the normal group,the levels of serum AST,ALT,HA,LN and PC Ⅲ were significantly elevated,and the protein expression levels of TGF-β1,and α-SMA in hepatic tissues were significantly increased in the model group. As compared with the model group,the levels of serum ALT,AST,HA,LN,PC III were significantly decreased in SCFCE groups. Histopathological test results showed that SCFCE groups could significantly improve the heptic histological structure of rats,decrease the degree of liver fibrosis,and significantly reduce the protein expression levels of TGF-β1,α-SMA（ P〈0. 01）. Conclusion： SCFCE has obvious protective effects on CCl4 and alcoholinduced liver fibrosis in rats,and its mechanism may be related to decreasing the protein expression levels of TGF-β1,α-SMA,and inhibiting the proliferation of hepatic stellate cells.

关 键 词：五味子 藤茎提取物 转化生长因子-β1 Α-肌动蛋白 

分 类 号：R285.5[医药卫生—中药学]