12产地甘草中18α-甘草酸及18β-甘草酸的含量分析  

Analysis of 18α- and 18β-Glycyrrhizic Acid Contents in Glycyrrhiza uralensis Fisch. from 12 Different Habitats

作　　者：马永生[1] 杨瑞[1] 袁伯川 周姗[1] 李文东[2] 刘颖[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京市药品检验所,北京100035

出　　处：《生物技术通讯》2016年第4期520-524,共5页Letters in Biotechnology

基　　金：国家自然科学基金(81508131)

摘　　要：目的：分析不同产地甘草中18α-甘草酸及18β-甘草酸的含量差异,为不同产地甘草的质量评价提供参考。方法：采集7个省区12个不同产地的甘草种子,栽培于北京中医药大学药草园,一年后以其中180株甘草作为实验材料,利用内转录间隔区（ITS）序列鉴定其基原,采用高效液相色谱（HPLC）法测定其18α-甘草酸及18β-甘草酸含量,并分析其差异性及相关性。结果：ITS鉴定结果显示180株甘草样品均为乌拉尔甘草。HPLC分析结果显示,18α-甘草酸的标准曲线为y=6×10-7x-0.0029（R2=0.9982）,在0.0111~0.2214μg范围内线性良好;18β-甘草酸的标准曲线为y=1×10-6x＋0.0164（R2=0.9999）,在0.2256~4.5120μg范围内线性良好。12个产地中山西应县甘草样品的18α-甘草酸及18β-甘草酸含量均最高,新疆尼勒克县甘草样品的18α-甘草酸及18β-甘草酸含量均最低,且所有样品中18α-甘草酸与18β-甘草酸的含量均存在显著相关关系。结论：不同产地甘草质量差异显著,本实验结果可为不同产地甘草的质量评价提供参考。Objective： To investigate the quality of Glycyrriza uralensis Fisch. from 12 different habitats by ana-lyzing the contents of 18α-glycyrrhizic acid（18α-GA） and 18β-GA. Methods： Seeds of G.uralensis were obtainedfrom 12 habitats and cultivated in the herbal garden of Beijing University of Chinese Medicine for one year. Total-ly 180 G.uralensis plants（15 plants/habitat） were collected as samples, which were identified by internal tran-scribed spacer（ITS） sequence, and then the contents of 18α-GA and 18β-GA in which were analyzed by HPLC.Results： ITS identification results showed that all of the samples were G.uralensis. HPLC results showed that thecalibration curves for 18α- GA and 18β- GA were y=6 × 10- 7x- 0.0029（R2=0.9982） and y=1 × 10- 6x ＋ 0.0164（R2=0.9999）, respectively. The calibration curves reflected a good linearity in the range of 0.0111~0.2214 μg for 18α-GA and 0.2256~4.5120 μg for 18β-GA. The contents of 18α-GA and 18β-GA were both the highest in Yingx-ian, Shanxi province and the lowest in Nileke, Xinjiang Uygur Autonomous Region. In addition, the content of18α-GA was strikingly related to the content of 18β-GA. Conclusion： The quality of G.uralensis from differenthabitats varied a lot. This paper will provide a basis for quality evaluation of G.uralensis from different habitats.

关 键 词：乌拉尔甘草 18Α-甘草酸 18Β-甘草酸 高效液相色谱法 含量分析 

分 类 号：Q943[生物学—植物学] R284[医药卫生—中药学]