兔自体富血小板血浆对兔ACL重建后腱骨愈合的影响  

Effect of platelet rich plasma on the healing of tendon bone after reconstruction of rabbit ACL

作　　者：李洋[1] 徐斌[1] 徐洪港[1] 涂俊[1] 杨军军[1] 

机构地区：[1]安徽医科大学第一附属医院运动创伤与关节镜外科,合肥230032

出　　处：《安徽医科大学学报》2016年第9期1277-1280,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1208085MH157)

摘　　要：目的探讨兔自体富血小板血浆(PRP)对前交叉韧带(ACL)重建后腱骨愈合的作用。方法选取30只新西兰大白兔,兔龄体重相仿,随机均分为实验组和对照组。实验组每只抽取自体全血,二次离心法获得PRP。实验组移植入PRP和生物蛋白凝胶复合物;对照组移植入生物蛋白凝胶。分别于4、8、12周3个时间点处死5只兔子取材,3只用于骨道内移植物抗拉力试验,采用独立样本t检验比较;2只用于观察形态学,对上述3个时间点的两组标本分别行腱骨界面Buark评分,采用Wilcoxon秩和检验,观察ACL重建术后腱骨愈合的情况。结果 Masson三色染色显示实验组术后8周前胶原纤维增生不明显,排列混乱,8周后开始到12周胶原纤维开始大量增生,排列逐渐有序;而对照组8周及12周只见到少量排列无序的胶原纤维。移植物抗牵拉实验显示实验组和对照组的股骨道移植物抗牵拉强度与时间长度呈正比,由8周开始PRP组明显强于对照组(P<0.01)。结论PRP对腱骨界面胶原纤维形成以及生物力学强度有促进作用。Objective To investigate the effect of autologous platelet rich plasma（ PRP） in tendon bone healing after reconstruction of the anterior cruciate ligament（ ACL）. Methods 30 New Zealand rabbits were randomly divided into the experimental group and the control group（ n = 15 per group）. In the experimental group the platelet rich plasma was obtained by the method of two-step centrifugation,and the experimental group was implanted with PRP and fibrin gel and the control group received gel only. The 5 rabbits in each group were retrieved at 4th,8th and 12 th week,All animals for gross observation at first,then three rabbits were used for biomechanical testing,Two rabbits for the pathological observation,the staining results were scored using the BUARK. Results 8 weeks after operation,the collagen fiber hyperplasia was not obvious in the experimental group,and the arrangement was in disorder. From the 8th week to the 12 th week of the experimental group,the collagen fibers began to proliferate,the arrangement became more ordered. In the control group,only a small amount of collagen fibers were found in the 8th and 12 th week. In the experimental group and the control group,the maximum biomechanical pull-out strength of the autograft in the femur tunnel was proportional to the repair time,and the experimental group was significantly stronger than that in the control group at the beginning of 8th weeks（ P〈 0. 01）. Conclusion PRP can promote the formation of collagen fibers and the mechanical strength of the tendon bone interface.

关 键 词：前交叉韧带 富血小板血浆 腱骨愈合 

分 类 号：R686.5[医药卫生—骨科学]