超声成像技术在大西洋鲑早期性别及发育期鉴别的应用研究  

The application of ultrasound imaging technology in the early determination of sex and gonadal development in Atlantic salmon(Salmo salar)

作　　者：仇登高[1,2,3] 徐世宏[1,3] 刘鹰[1,3,4] 宋昌斌[5] 迟良[1] 王顺奎 于凯松 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]福建省水产研究所福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013 [3]海洋生态养殖技术国家地方联合工程实验室,山东青岛266071 [4]大连海洋大学,辽宁大连116023 [5]中国科学院半导体研究所,北京100083 [6]山东东方海洋科技股份有限公司,山东烟台264003

出　　处：《海洋科学》2016年第6期23-29,共7页Marine Sciences

基　　金：国家自然科学基金项目(31472312;41306152;31402283);国家"十二五"科技支撑计划项目(2014BAD08B09);江苏省重点研发项目(BE2015325)~~

摘　　要：采用KX-5100便携式B超机（7.5MHz高频线阵探头）对273尾1龄或2龄大西洋鲑（Salmo salar）的性别及性腺发育阶段进行了检测,同时对检测的大西洋鲑进行了解剖和取样,利用组织学观察法验证了超声成像技术对大西洋鲑雌雄性别和发育阶段的鉴别结果。研究结果显示：利用超声成像技术鉴别雌、雄大西洋鲑的平均准确率为86%,且鉴别雌鱼的准确率（93%）较雄鱼（81%）高。超声成像技术鉴别Ⅱ期＋Ⅲ期、Ⅳ期、Ⅴ期卵巢的准确率分别为87%、92%和100%,鉴别Ⅱ期、Ⅲ期、Ⅳ期、Ⅴ期精巢的准确率分别为64%、71%、86%和84%。总体看来,超声成像技术鉴别早期性腺（Ⅱ期~Ⅲ期）发育的准确率较低,仅为75%,而对晚期性腺（Ⅳ期~Ⅴ期）发育鉴别的准确率较高,达到89%。Cultured Atlantic salmon（n = 273）, aged 1–2 years were examined using an ultrasound device（KX-5100; with a 7.5-MHz linear probe） from April to December of 2012. Following ultrasound scanning, necropsy and sampling were performed and histological specimens were prepared to independently confirm ultrasound determinations of sex and developmental stages. Results showed that, on average, the accuracy of identification using ultrasound imaging technology was 86% in Atlantic salmon, and there was a higher accuracy of sex identification of females（93%） than males（81%）. In addition, the accurate identification of stages Ⅲ, Ⅳ and Ⅴ in the ovaries of female fish were 87%, 92% and 100%, respectively, while the accurate identification of stages Ⅱ, Ⅲ, Ⅳ and Ⅴin male testes were 64%, 71%, 86% and 84%, respectively. Overall, the accuracy of gonadal development in early stages（i.e., stages Ⅱ and Ⅲ） was lower（75%） than that of the later stages（i.e., stages Ⅳ and Ⅴ）, which was 89%.

关 键 词：超声成像技术 大西洋鲑(Salmo salar) 早期性别鉴别 性腺发育阶段 

分 类 号：Q955[生物学—动物学]