原癌基因PIM-2转录蛋白生物信息学分析  

Bioinformatics Analysis of Proto-oncogene PIM-2 Transcription Protein

作　　者：周小东[1] 郑崛村[1] 

机构地区：[1]成都医学院检验医学院,成都610500

出　　处：《河南师范大学学报（自然科学版）》2016年第1期142-148,179,共8页Journal of Henan Normal University(Natural Science Edition)

基　　金：成都医学院校基金(CYZ11-022);2012年四川省卫生厅科研资助项目(120499)

摘　　要：目的:研究原癌基因PIM-2的抗凋亡、致癌机理,用生物信息学的方法分析转录蛋白,为临床应用提供科学依据和理论基础.方法使用pbil,expasy,RasMol,DNAStar Lasergene等生物信息学在线服务器和相关软件,对PIM-2转录蛋白进行全面分析预测.结果:成功预测出PIM-2转录蛋白的空间结构,各种理化性质,该蛋白是一种不稳定的酸性蛋白,序列上分布着17段B细胞抗原表位,14段Th细胞抗原表位,3段CTL细胞表位,多段MCH I分子结合肽和MCH II分子结合肽.结论:PIM-2转录蛋白的结构、表位、特性、修饰具有重要意义,可为深入研究原癌基因Pim-2的作用机理提供科学依据和理论帮助.Objective：To study the antiapoptosis and carcinogenesis mechanism of proto-oncogene PIM-2, and analyze the transcription protein from bioinformatics aspect, so as to provide scientific and theoretic basis for clinical practice. Method：To comprehensively analyze and predict proto-oncogene PIM-2 transcription protein with online bioinformatics servers and related software, such as pbil, expasy, RasMol, DNAStar Lasergene. Result.. the protein spatial structure and physicochemical charac- teristics of PIM-2 are predicted. The protein is turned to be an destabilized acidic protein, with 17 B Cell epitopes, 14 Th cell epitopes, 3 CTL epitopes, and several MCH class I ＆ II peptide bindings in the sequence. Conclusion.. The structure, epitope, character, modification of PIM-2 transcription protein have great significance, which can provide scientific basis and heoritical support for further research on proto-oncogene PIM-2 transcription protein mechanism of action.

关 键 词：原癌基因 PIM-2 生物信息学 结合肽 

分 类 号：Q811.4[生物学—生物工程]