不同光质LED光源对转基因杨树叶片Bt毒蛋白含量及叶绿素荧光参数的影响  

Effect of Different LED Light Qualities on Bt Toxic Protein Content and Chlorophyll Fluorescence of Transgenic Poplar Leaves

作　　者：张文林[1] 任亚超[1] 张益文[1] 刘红梅[1] 张军[1] 

机构地区：[1]河北农业大学林学院,河北保定071000

出　　处：《核农学报》2016年第8期1639-1645,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)项目(2013AA102703)

摘　　要：为探明不同光质对杨树幼苗生长的影响,以转基因741杨株系Pb29为试材,利用LED精量调制光源,设置红光、蓝光、红蓝光、白光4个处理,测定不同光质处理下Pb29的Cry1Ac毒蛋白含量、形态指标、光合色素含量及叶绿素荧光参数。结果表明,蓝光处理下的Cry1Ac毒蛋白的表达量最大,白光次之。除了叶片长/宽的值在蓝光处理下最大外,红蓝光处理下的741杨叶片数、叶片长、叶片宽、叶面积及株高均高于其它处理。除叶绿素b的含量在红光处理下最大外,叶绿素a、叶绿素a+b、类胡萝卜素含量及叶绿素a/b比值均在白光处理下最大,红蓝光次之,且白光与红蓝光间无显著差异。叶绿素荧光参数(ΦPSⅡ、ETR、q P)在蓝光和红蓝光处理下高于其它处理,在白光和红蓝光处理下的Fv/Fm高于其它处理,q N在红光处理下最大。由此可知,不同光质对杨树幼苗的生长具有调控作用。本试验结果为深入研究不同光质对杨树的生长发育进行调控提供了科学依据。To clarify the effect of different light quality treatment on the growth of polar, transgenic poplar line Pb29 was taken as experimental material, and full red light, full blue light, red and blue light, full white light four treatments were set by use of light emitting diode （LED） to measure the content of CrylAc toxic protein, morphological index, photosynthetic pigment content and chlorophyll fluorescence parameters of Pb29 under different light qualities. The results showed that the content of Cryl Ac toxic protein is the highest under the blue light and followed by the white light. Apart from the fact that leaf length/width is the highest under the blue light, the leaf number, leaf length, leaf width, the area of leaf and plant height are the highest under the red and blue light. While the content of chlorophyll b is the highest under the red light treatment, the content of chlorophyll a, chlorophyll a ＋ b, carotenoid and chlorophyll a/b ratio are the highest under the white light treatment and followed by the red and blue light, and no significant difference was observed between white light and red/blue light. The chlorophyll fluorescence parameters （ФPSⅡ , ETR and qP） are higher than others under the blue light and red/blue light treatments, but the Fv/Fm is greater than others under the white light and red/blue light treatments respectively. While qN is the highest under the red light treatment. Thus it can be seen that different light quality can regulate the growth of the poplar seedlings. The result in this present study will provide a scientific basis for further study on the effect of different light quality on regulating the growth and development of poplar.

关 键 词：741杨 光质 Cry1Ac毒蛋白 光合色素 叶绿素荧光 

分 类 号：S792.11[农业科学—林木遗传育种]