重离子辐射诱导水稻DNA甲基化变化的分析  

DNA Methylations of Rice Seeds Induced by Heavy Ion Radiation

作　　者：赵倩[1] 王巍[1] 张萌[1] 史金铭[2] 韩璐[1] 孙野青[1] 

机构地区：[1]大连海事大学环境科学与工程学院/环境系统生物学研究所,辽宁大连116026 [2]东北林业大学生命科学学院,黑龙江哈尔滨150040

出　　处：《核农学报》2016年第9期1665-1671,共7页Journal of Nuclear Agricultural Sciences

基　　金：中央高校基本科研业务费专项资金(3132013089);中央高校基本科研业务费专项资金(3132014103);中国科学院空间科学战略性先导科技专项(XDA04020202-12)

摘　　要：为了研究不同剂量重离子辐射对植物产生的表观遗传学效应规律,采用2种不同的高传能线密度和不同剂量(0、0.2、2Gy)的12C6+放射流辐照干燥的水稻种子,分别选取辐射水稻三叶期及分蘖期的叶片,通过甲基化敏感扩增多态性技术(MSAP)进行CCGG位点的甲基化状态分析。结果表明,重离子辐射,尤其是高剂量的辐射明显造成水稻DNA甲基化的多态性水平的变化,其中DNA去甲基化的多态性水平明显高于DNA甲基化的多态性水平,且DNA甲基化位点更倾向于CNG。此外,利用全基因组甲基化测序进一步验证了重离子辐射对水稻DNA甲基化的影响。本研究结果为探讨空间辐射引起水稻表观遗传变化的分子机制提供了重要的理论依据。To investigate the epigenetic effects of different doses of heavy ion radiation on plants, dry rice seeds were exposed to 12^C6^＋heavy ion radiation with different doses （0, 0.2, 2Gy）. Leaves of plants at seeding stage and tillering stage were obtained for DNA methylation analysis by methylation-sensitive amplified polymorphism （MSAP）. Results showed the epigenomic alterations were induced by heavy ion radiation in rice, especially for high-dose, the effects on demethylation was more obvious than those on hypermethylation, and DNA methylation changes tended to occur on CNG site rather than CG site. The effects of heavy ion radiation on DNA methylation were further anfirmed by methylome sequence analysis from rice exposed to the same radiation doses. This research will provide an important evidence for the molecular mechanism of the epigenetic in rice induced by radiation in space.

关 键 词：水稻 重离子辐射 DNA甲基化 MASP BS-Seq 

分 类 号：S511[农业科学—作物学]