南方型紫花苜蓿叶片响应盐胁迫蛋白质组和转录组关联分析  

Correlation Analysis on Proteomic and Transcriptomic of Salt-response in Leaves of Southern Type Alfalfa

作　　者：马进[1] 郑钢[1] 裴翠明 张振亚[1] 

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江临安311300

出　　处：《核农学报》2016年第9期1706-1715,共10页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31272494);浙江省自然科学基金(LY16C170003)

摘　　要：为了从基因和蛋白质水平上揭示南方型紫花苜蓿适应盐胁迫环境的分子机制,以南方型紫花苜蓿Millennium为材料,对正常培养和盐胁迫条件下的2个样品叶片进行转录组和蛋白质组关联分析。结果表明,定量蛋白和基因关联系数为0.2485;变化趋势相反差异蛋白和基因表达的关联系数为-0.2440;变化趋势相同差异蛋白质和基因表达的关联系数为0.8122。鉴定出109个与差异基因表达趋势相同的差异蛋白,其中77个上调,32个下调,这些差异蛋白功能涉及光合作用、抗氧化物、信号传递、翻译后修饰、翻译和分子伴侣、胁迫防御、能量产生与转运、代谢和其它未知功能蛋白等。下调表达的蛋白主要与光合作用相关,而上调表达的蛋白主要参与了抗氧化物、信号传递和胁迫防御等。此外,关联发现了与紫花苜蓿盐胁迫响应相关的III类过氧化物酶、铁蛋白、谷胱甘肽S-转移酶、磷脂酰肌醇特异性磷脂酶C、LRR类受体激酶、ABA反应蛋白、钙联接蛋白2、液泡H+-ATP酶C亚基和NADP-苹果酸酶等差异蛋白。本研究通过高通量多组学数据的关联分析,发现一些可能作为紫花苜蓿耐盐潜在靶标蛋白(基因),这为深入认识紫花苜蓿盐胁迫的应答分子调控机制奠定了坚实的基础。Integrative analysis of multi-omics data would provide innovative perspectives on the complex mechanisms of plant adaptation to salt stress. In order to investigate the molecular mechanism of salt tolerance, the transcriptomes and proteomie of southern type Alfalfa （Medicago sativa） ＂ Millenium＂ leaves under control condition were compared with those under NaCl-treated condition. The results indicated that the correlations between the quantitative protein and mRNA differentially expressed proteins and mRNA expression with opposite expression trend, differentially expressed proteins and mRNA with same expression trend were 0. 2485, -0. 2440 and 0. 8122, respectively. The number of the differential expression proteins which were expressed at the same trend with differential expression genes was 109, among which 77 and 32 were detected to be up-and down-regulated, respectively. These differential expression proteins were revealed to be involved in various biological processes such as photosynthesis, oxidation resistance, signal transmission, posttranslational modification, protein turnover, chaperones, defense against stress, energy and transport, metabolism and other as well as function unknown proteins. The down regulated proteins were principally involved in photosynthesis, while the up regulated proteins were oxidation resistance, signal transmission and defense against stress. Candidate differential expression proteins closely related to salt stress resistance, such as class III peroxidase, ferritin, lutathione S- transferase, phosphatidylinositol-specific phospholipase C, ABA-responsive protein, heat shock 70 kDa protein, ealnexin 2, vacuolar H ^＋ -ATPase subunit C and NADP-malic enzyme were selected through correlation analyses proteomie and transcriptomic. Through integrative analysis of multi-omics data, the potential target proteins （genes） found in this paper would lay a solid basis for a holistic understanding of the molecular regulatory mechanism in alfalfa in response to salt stress.

关 键 词：南方型紫花苜蓿 盐胁迫 蛋白质组 转录组 关联 

分 类 号：S541.9[农业科学—作物学]