油菜种子低亚油酸和亚麻酸含量异交稳定技术的建立  

Establishment of a Technique for Stable Maintenance of Low Linoleic and Linolenic Acid Contents in Outcrossed Seeds of Rapeseed

作　　者：郎春秀[1] 王伏林[1] 吴学龙[1] 石江华[1] 陈锦清[1] 吴关庭[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《核农学报》2016年第9期1716-1721,共6页Journal of Nuclear Agricultural Sciences

基　　金：浙江省自然科学基金重点项目(Z306077)

摘　　要：为降低油菜种子中的亚油酸和亚麻酸含量并使之在异交条件下保持基本稳定,以高油品种CY2作为受体亲本,采用RNA干涉技术沉默油菜内源Bn FAD2基因的表达,抑制油酸脱饱和反应。育成的3个独立转基因株系的亚油酸和亚麻酸含量下降到6%以下,其中亚油酸含量较受体亲本降低78.2%-86.5%,亚麻酸含量降低53.4%-65.8%。将3个转基因株系与2个亚油酸和亚麻酸含量较高的常规品种正反交,F1种子中这2种脂肪酸的含量依然保持在与转基因亲本相仿的低水平。由此可知,通过RNA干涉技术沉默Bn FAD2基因的表达可赋予油菜低亚油酸和亚麻酸含量的异交稳定特性。这一特性的获得对于今后培育异交稳定的高油酸油菜新品种具有重要意义。In order to decrease linoleic and linolenic acid contents and to make them stable in Brassica napus seeds, the endogenous BnFAD2 gene in a high oil cuhivar CY2 was knocked down by RNA interference （RNAi） to inhibit desaturation reaction of oil acid. Three independent transgenic lines with linoleic and linolenic acid contents lower than 6% were obtained. Compared with CY2, the contents of linoleic acid and linolenic acid in these lines were decreased by 78.2% -86. 5% and 53.4% -65.8% , respectively. In F1 hybrid seeds derived from reciprocal crossing between the transgenic lines and two high linoleic and linolenic cuhivars, the contents of linoleic and linolenic acids still remained similar low levels as those in transgenic parents. These results indicate that rapeseed can be endowed with an ability to stably maintain low linoleic and linolenic acid contents by RNAi-mediated silencing of BnFAD2 gene, which will has important significance for accumulating and stably maintaining high oleic acid content in outcrossed Brassica napus cultivars.

关 键 词：油菜 亚油酸 亚麻酸 FAD2基因 RNA干涉 

分 类 号：S565.4[农业科学—作物学]