耐盐产氢菌C.beijerinckii n232的分离及产氢能力研究  

Study on the isolation of a NaCl tolerance biohydrogen production bacterium C.beijerinckii n232 and its hydrogen production capacity

作　　者：许淑红[1] 赵鑫[1] 李丹阳[1] 李雪洁[1] 曹天舒[2] 胡筱敏[1] 

机构地区：[1]东北大学资源与土木工程学院,辽宁沈阳110819 [2]辽宁省环境保护宣传教育中心,辽宁沈阳110033

出　　处：《可再生能源》2016年第8期1252-1258,共7页Renewable Energy Resources

基　　金：国家自然科学基金项目(51408103);教育部青年教师创新基金项目(N140104005);辽宁省自然科学基金项目(2015020601)

摘　　要：从秦皇岛海滨泥沙样品中分离获得一株具有耐盐性的产氢细菌,编号为n232。通过生理生化鉴定和16S r DNA系统发育分析,确定该菌株为拜氏梭菌的新菌株,命名为Clostridium beijerinckii n232(KU750806)。菌株n232发酵产氢的最适碳源为葡萄糖,葡萄糖的最适初始浓度为10 g/L;最适初始p H为7.0;蛋白胨与酵母粉的最佳投加量分别为3 g/L和2 g/L;投加NaCl可以促进细胞生长,提高比产氢率。使用优化的培养基,发酵24h,菌株n232获得的最大细胞干重为1.13 g/L,最大比产氢率为2.14 mol/mol。耐盐发酵产氢细菌的筛选为高盐度有机废水资源化提供了新的思路。A hydrogen-producing strain with NaC1 tolerance capacity numbered n232 was isolated from Qinhuangdao coast sediment sample. It was identified belonging to Clostridium beijerinckii based on 16S rDNA sequencing and physiological-biochemical analysis, and named as Clostridium beijerinckii n232 （KU750806）. The experiments results indicated that glucose was the most suitable carbon source, the optimum initial concentration of glucose was 10 g/L, the favorite initial pH was 7.0, the most suitable concentration of peptone and yeast powder were 3 g/L and 2 g/L, and adding suitable concentration NaC1 had a promoting effect on cell growth and hydrogen yield of strain n232. The maximum dry cell weight of 1.13 g/L and hydrogen yield of 2.14 tool/tool were obtained with the optimal fermentative culture medium after 24 h fermentation. The isolation of stdt tolerance hydrogen producing bacteria provides a new idea for the high salinity organic wastewater resources.

关 键 词：生物制氢 产氢细菌 耐盐菌拜氏梭菌 培养基优化 

分 类 号：TK6[动力工程及工程热物理—生物能] Q93-3[生物学—微生物学]