Undifilum oxytropis次级代谢产物GC-MS分析及抗肿瘤活性研究  

GC-MS and antitumor activity study on the secondary metabolites of Undifilum oxytropis

作　　者：刘忠艳[1] 薛瑞旭 吴庆侠[1] 董海龙[1] 李鹏[3] 金艺鹏[3] 林德贵[3] 

机构地区：[1]西藏农牧学院,西藏林芝860000 [2]西北农林科技大学动物医学院,陕西杨凌712100 [3]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2016年第7期19-21,共3页Chinese Journal of Veterinary Medicine

基　　金：中国农业大学基本科研业务经费专项资金(2015-TC027);西藏自治区科技厅自然科学基金(2015ZR-13-31)

摘　　要：研究疯草内生真菌(Undifilum oxytropis),次级代谢产物成分与活性,通过GC-MS对菌丝体及发酵液进行了分析,并通过MTT法测定抗肿瘤活性。结果表明,菌丝体和发酵液中均以烷烃和酯类物质为主,菌丝体中检出活性物质角鲨烯(0.863%);菌丝体对HCT-8人回盲肠癌细胞和OS-RC-2人肾癌细胞抑制率均较高,发酵液对HCT-8人回盲肠癌细胞抑制率相对较弱。In order to study the active ingredients of secondary metabolites in locoweed endophyte,Undifilum oxytropis,themycelium and fermentation broth were analyzed by GC-MS,and antitumor activity was measured by MTT assay.It showed that：al-kanes and esters were mainly active substances in both mycelium and fermentation broth. And squalene（0.863%）was detected inthe mycelium;HCT-8 human ileocecal cancer and OS-RC-2 human renal cancer were highly inhibited by the mycelium.Inhibi-tion rate of fermentation broth to HCT-8 human ileocecal cancer was relatively weak.

关 键 词：疯草 内生真菌 代谢产物 抗肿瘤 

分 类 号：S856.9[农业科学—临床兽医学]