UNG酶在兽用生物制品支原体PCR检测中防止假阳性的应用  

Application of Uracil-N-glycosylase to Avoid False Positive in the Detection of Mycoplasma Contaminate in Veterinary Biological Products

作　　者：郭建华[1] 李向碧 岳秀阳 曹政 

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460 [2]重庆澳龙生物制品有限公司,重庆荣昌402460

出　　处：《中国兽医杂志》2016年第7期83-85,共3页Chinese Journal of Veterinary Medicine

基　　金：西南大学科研基金资助(08BSr04)

摘　　要：为了研究尿嘧啶-N-糖基化酶(UNG酶)在减少兽用生物制品支原体PCR检测中的假阳性情况,本研究通过在PCR试剂中加入UNG酶,在PCR反应前25℃作用10 min,再50℃作用2 min,结果显示,该法可以有效防止PCR产物的污染,减少假阳性的产生。To investigate the method of avoidance of false positive in the detection of mycoplasma contaminate in veterinary bi-ological productions,Uracil-N-glycosylase was added to PCR reaction mix.And 25 ℃ for 10 minutes and 50 ℃ for 2 minutes wereprocessed before PCR reaction.The results showed that uracil-N-glycosylase prevented the contamination of PCR products and re-duced the false positive efficiently.Uracil-N-glycosylase can avoid false positive in detection of mycoplasma contamination in vet-erinary biological production.

关 键 词：UNG酶 兽用生物制品 PCR 假阳性 

分 类 号：TQ464.8[化学工程—制药化工]