组织工程化脂肪填充材料的构建及脂肪颗粒存活研究  

Construction of tissue engineering fat filler and survival of fat particles

作　　者：崔永言[1] 刘高峰[1] 陈萍[2] 庄礼大[1] 朱辉[1] 

机构地区：[1]北京大学深圳医院整形外科,518036 [2]北京大学深圳医院计划生育科,518036

出　　处：《中华医学美学美容杂志》2016年第4期231-235,共5页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：深圳市科技计划项目（JCYJ0140415162543014）

摘　　要：目的研究组织工程化脂肪填充材料的构建及脂肪颗粒存活情况。方法从兔脂肪组织提取血管基质部分（SVF）混合自体成熟脂肪颗粒，加或不加入生长因子神经肽Y（NPY），构建聚乳酸-羟基乙酸共聚物（PLGA）组织移植复合体，并移植到兔背部皮下，同时进行对照实验。按不同组合分5组：A组为PLGA＋成熟脂肪颗粒＋无NPY小球；B组为PLGA＋成熟脂肪颗粒＋NPY小球；C组为PLGA＋SVF与成熟脂肪颗粒混合物＋无NPY小球；D组为PLGA＋SVF与成熟脂肪颗粒混合物＋NPY小球；E组为SVF与成熟脂肪颗粒混合物＋NPY小球。于2周、1个月、3个月后通过大体观察、组织学、荧光示踪等方法来评价不同构建方式的差异及脂肪颗粒的存活情况。结果D组1个月、3个月时在存活体积、质地、再血管化程度方面均优于A组、B组、C组、E组，脂肪平均存活率（57．5±2．5）%，脂肪细胞生长良好，前体细胞增殖分化活跃。结论以SVF，成熟脂肪颗粒，PLGA和NPY成功构建了性能良好的组织工程材料，明显提高脂肪颗粒移植效果。Objective To study the construction of tissue engineering fat filler and the survival of fat particles. Methods Stromal vascular fraction （SVF） was derived from inguinal subcutaneous fat of rabbits, mixed with autologus mature fat particles and with or without neuropeptide Y to con- struct PLGA composite tissue. The complex was subcutaneously transplantef into back sites of the rabbits. Based on the different combination five groups were divided： Group A： PLGA ＋ mature fat particles without NPY; Group B： PLGA ＋ mature fat particles ＋ NPY; Group C： PLGA ＋ com- plex of SVF with mature fat particles without NPY; Group D： PLGA ＋ complex of SVF with mature fat particles ＋ NPY; Group E： Complex of SVF with mature fat particles ＋ small ball with NPY. Diffenrence of virous constructive ways and fat particle survival was evaluated by general observation, histological staining, fluorescence tracing at two weeks, one month and three month after operation. Resnlts Group D was superior to groups A, B, C and E in survival volume, graft texture and vascularization at one mouth and three mouths. The fat average srvival rate was 57.5±2.5%. Fat cell grew well, and precursor cells proliferated and differentiated actively. Conclusions High quality tissue engineering materials are successfully established with SVF-mature fat particle complex, PLGA and NPY, which could obviously improve fat particle transplantation.

关 键 词：脂肪移植 组织工程 血管基质部分 神经肽Y 

分 类 号：R622.9[医药卫生—整形外科]