猪源大肠杆菌tem耐药基因的检测  

Detection of the drug-resistant gene tem from swine-derived Escherichia coli

作　　者：史秋梅[1] 项方 郭蕊 张召兴[1] 张艳英[1] 刘玉芹[1] 杨彩然[1] 杨宗泽[1] 高光平[1] 高桂生[1] 刘立帅 

机构地区：[1]河北省预防兽医学重点实验室/河北科技师范学院,河北昌黎066600 [2]昌黎县动物疾病预防控制中心,河北昌黎066600

出　　处：《黑龙江畜牧兽医（下半月）》2016年第8期106-108,共3页

基　　金：科技部农业成果转化项目(2012GB2A200045);河北省科技厅科技支撑计划项目(10960408D);河北省科技厅奖励性后补助项目

摘　　要：为了解和掌握大肠杆菌耐药性的流行情况,从而为控制大肠杆菌的耐药性提供科学依据,用K-B法对临床分离的15株猪源大肠杆菌进行β内酰胺类抗生素药敏试验,以15株分离菌DNA为模板对β内酰胺类抗生素耐药基因tem进行PCR扩增。结果表明:15株猪源大肠杆菌对β内酰胺类抗生素均耐药,耐药率从高到低分别为头孢哌酮(82%)、头孢噻肟(24%)、头孢他啶(18%)、头孢呋辛(6%)、苯唑西林(6%);PCR扩增均检测到β内酰胺类抗生素耐药基因tem。说明β内酰胺类抗生素耐药基因tem广泛存在于猪源大肠杆菌中,与抗生素敏感性表型一致。To understand and master the pandemic situation of drug resistance of Escherichia coli （E. coil）, thereby providing a scientific basis for controlling its drug resistance, a drug sensitivity test for β - lactam antibiotics was conducted using K - B method for fifteen clinical isolates of swine - derived E. coll. The tern gene as the drug - resistant gene of β - lactam an oxacillin tibiotics was amplified by PCR taking DNAs of the fifteen isolates as templates. The results showed that the fifteen isolates of swine - derived E. call were resistant to β - laetam antibiotics, and the drug resistance rates for β - lactams antibiotics in＇a descending order were as follows： cefoperazone （82%） , cefotaxime （24%）, ceftazidime （ 18% ） , .cefuroxime （6%） and oxaeilhn （6%）. Meanwhile, the tern genes were detected in swine - derived E. coli by PCR. The results indicate that the tern gerne as the drug - resistant gene of β - lactam antibiotics is widely existed in swine - derived E. coli, which is consistent with the sensitivity phenotype of antibiotics.

关 键 词：猪源大肠杆菌 耐药性 药敏试验 tem耐药基因 β内酰胺类抗生素耐药基因 PCR扩增 

分 类 号：S852.612[农业科学—基础兽医学]