分子标记辅助选择抗南方玉米锈病材料  

Selection of materials with resistance to southern corn rust(SCR) by marker-assistant selection(MAS) in maize( Zea mays L.)

作　　者：谭华[1] 邹成林[1] 郑德波[1] 韦新兴[1] 黄爱花[1] 蒋维萍[1] 韦慧[1] 黄开健[1] 

机构地区：[1]广西农业科学院玉米研究所,广西南宁530007

出　　处：《广东农业科学》2016年第7期6-10,共5页Guangdong Agricultural Sciences

基　　金：广西重大科技计划专项(桂科重14121001-1-9);广西自然科学基金(2013GXNSFAA019101)

摘　　要：以来自国际玉米小麦改良中心(CIMMYT)的高抗南方玉米锈病种质为抗锈源,把抗病基因导入来自温带欠抗锈种质,借助分子标记辅助选择,聚合抗锈基因定向改良,对获得的BC2F2进行田间抗病性接种鉴定。结果显示,利用phi048选择与抗病亲本相同带型的BC2F2家系368个单株接种鉴定,抗及高抗级别植株共235株,占总株数的63.9%;中抗级别植株114株,占31.0%;感病植株19株,占5.1%。表明利用phi048对南方玉米锈病抗病材料进行早代选择是有效的,可淘汰大量感病家系,减轻田间工作量;利用分子标记辅助定向选择结合抗病接种鉴定,能有效改良温带种质的抗锈性。In order to improve the maize resistance to southern corn rust of temperate germplasm,the resistant genes,which came from germplasm of high SCR-resistant in CIMMYT,were introduced in temperate germplasm. The SCR-resistance of temperate germplasm was directionaly improved by pyramiding SCR-resistant genes. 368 plants of a BC2F2 family,which had the same polymorphism with the SCR-resistant parent by detecting with SSR marker phi048,were used to identify their SCR-resistance by artificial inoculation in field. The results showed that 235 plants were highly resistant or resistant,accounting for 63.9%,114 plants were moderately resistant,accounting for 31.0%,19 plants were susceptible,accounting for 5.1%. The results demonstrated that SSR marker phi048 was effective to select SCR-resistant materials in early stage,which could knock out lots of SCR-susceptible lines therefore reduce plenty of field work. The SCR-resistance of temperate germplasm was effectively improved by directional selection with marker assistant combined to identification with artificial inoculation.

关 键 词：南方玉米锈病 分子标记辅助选择 抗锈改良 

分 类 号：S513.034[农业科学—作物学]