犏牛、牦牛睾丸组织中Ghrelin与GHSR基因mRNA表达水平研究  

Expression level of Ghrelin and GHSR mRNA in testis of cattle-yak and yak

作　　者：赵彦玲[1] 任子利[1] 李瑜鑫[1] 王建洲[1] 商鹏[1] 强巴央宗[1] 

机构地区：[1]西藏农牧学院动物科学学院,西藏林芝860000

出　　处：《广东农业科学》2016年第7期151-157,共7页Guangdong Agricultural Sciences

基　　金：西藏大学农牧学院2014年度高校生均奖补资金重点学科发展保障平台建设项目;西藏特色畜牧资源创新平台建设项目;西藏自治区自然科学基金(2015ZR-13-32)

摘　　要：对犏牛、牦牛睾丸组织中生长素(Ghrelin)与生长激素促分泌素受体(GHSR)基因mRNA表达水平进行研究。运用荧光定量PCR方法,检测Ghrelin、GHSR基因在犏牛、牦牛睾丸组织中m RNA的表达水平差异,结果显示:在犏牛、牦牛睾丸组织中,Ghrelin基因m RNA相对表达量犏牛显著高于牦牛(分别为0.8177±0.0225、0.4656±0.0222,P<0.05);GHSR基因mRNA相对表达量也是犏牛显著高于牦牛(分别为0.8622±0.0347、0.4722±0.0761,P<0.05)。相对于牦牛睾丸组织,犏牛睾丸组织中Ghrelin与GHSR基因m RNA均高表达可能是犏牛雄性不育的原因之一。The expression level of Ghrelin and growth hormone secretagogue receptor（GHSR） gene m RNA tested respectively in testis of cattle-yak and yak by using fluorescence quantitative polymerase chain reaction （PCR）. The results showed that Ghrelin m RNA expression level in cattle-yak testis was significantly higher than that in yak （0.8177±0.0225,0.4656±0.0222,P〈0.05）. Similarly,GHSR m RNA expression level in cattleyak testis was significantly higher than that in yak（0.8622±0.0347,0.4722±0.0761,P〈0.05）. These results indicated that both Ghrelin and GHSR m RNA expression level in cattle-yak testis was significantly higher than that in yak,which was likely to be one of the reasons for male infertility of cattle-yak.

关 键 词：犏牛 睾丸 生长素 生长激素促分泌素受体 MRNA表达 

分 类 号：S823.852[农业科学—畜牧学] Q344.13[农业科学—畜牧兽医]