一株铁皮石斛组织培养污染内生细菌的分离与鉴定  

Isolation and Identification of Pollution Endophytic Bacteria during Tissue Culture of Dendrobium candidum

作　　者：尹利方 陈泽斌[1,2] 夏体渊[1,2] 耿开友[3] 赵凤[3] 靳松[1] 李静雯[1] 牛燕芬[1] 

机构地区：[1]昆明学院农学院,云南昆明650214 [2]云南省都市特色农业工程技术研究中心,云南昆明650214 [3]昆明学院科研处,云南昆明650214

出　　处：《西南农业学报》2016年第8期1982-1986,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省都市特色农业工程技术研究中心项目(TSNY0201);国家自然科学基金项目(31460491);云南省科技厅基础研究项目(2013FD040);云南省教育厅科学研究项目(2014Y390);昆明学院大学生科研项目(XJD16081);昆明学院"人才引进项目"(YJL14005);云南省高校优势特色重点学科(生态学)建设项目(05000511311)

摘　　要：对污染铁皮石斛组培苗的内生细菌进行分离和鉴定,为预防铁皮石斛组织培养过程中内生细菌污染提供科学依据。采用NA培养基分离纯化细菌,通过形态特征、生理生化指标结合16S rDNA序列同源性分析,对引起铁皮石斛组培苗污染的内生细菌进行鉴定。引起铁皮石斛组培苗污染的内生细菌为SH42菌株,其形态特征及生理生化指标与莫海威芽孢杆菌(Bacillus mojavensis)基本相同;16S rDNA序列分析结果表明,SH42与B.mojavensis(AM948970)聚在同一系统发育分支,其同源性为99.4%。因此确定引起铁皮石斛组培苗污染的内生细菌SH42为莫海威芽孢杆菌。The advantaged endogenous bacteria, which caused contamination of Dendrobium candidum planflets, was isolated and identified so as to provide scientific references for pollution prevention during its tissue culture. Using the NA culture medium to isolate the strain, the bacterial species were identified by morphology, biochemical and the sequence analysis of the 16S rDNA. The results from physiological and biochemical tests showed that the morphological characteristics of the strain SH42 matched the descriptions of Bacillus mojaveusis. The se- quence analysis of 16S rDNA indicated that this strain had the same embranchment with the B. mojavensis （AM948970） in the phylogenetic tree with the homology of 99.4 %. The SH42 strain was identified as B. mojavensis.

关 键 词：铁皮石斛 组织培养 内生细菌 分离 鉴定 

分 类 号：S182[农业科学—农业基础科学]