马源抗犬瘟热病毒免疫球蛋白的制备  

PREPARATION OF HORSE IMMUNOGLOBULINS AGAINST CANINE DISTEMPER VIRUS

作　　者：严妍[1,2] 张锦霞[2] 王东方[1,2] 郑光来 张守峰[2] 扈荣良[2] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,长春130122

出　　处：《中国动物传染病学报》2016年第3期12-15,共4页Chinese Journal of Animal Infectious Diseases

基　　金：基金项目:新型动物疫病诊断技术应用研究(201203056)

摘　　要：将犬瘟热病毒(Canine distemper virus,CDV)疫苗株接种Vero细胞,连续培养至TCID50为10-4.625/m L。采用肋间肌多点注射方法免疫马,4周后每2周采集血液,分离血清,病毒中和试验测定血清中中和抗体效价,最高值达到212.5。以硫酸铵沉淀法纯化Ig G,病毒中和试验测定其中和抗体效价可达214。通过肌注犬观察,无明显不良反应。研究结果证明,本试验制备的马源免疫球蛋白可用于犬的抗体治疗,为犬瘟热的治疗制剂研发开辟了新方向。In the present study, the antiserum containing neutralizing antibodies produced in horses provided a feasible treatment for canine distemper. Production of Canine distemper viruses （CDVs） were conducted by inoculating and passaging the commercial CDV vaccine onto Veto cells until the virus titers reached to TCIDso of 10-4.625/mL. Four weeks after immunization of horses with CDVs, antiserum samples were obtained and afterwards harvested every two weeks. The immtmoglobulins （IgGs） were purified by ammonium sulfate precipitation. The pure IgGs were titrated to be 1：214 as measured for virus neutralizing activity. Five Beagle dogs were injected with the IgG preparation and no side-effects were observed, indicating its safety for canines.

关 键 词：犬瘟热病毒 纯化 免疫球蛋白 马 

分 类 号：S852.659.5[农业科学—基础兽医学]