PCV2与PRRSV体外共感染对肺泡巨噬细胞系炎症反应的影响  

INFLAMMATORY RESPONES OF A PULMONARY ALVEOLAR MACROPHAGE IN VITRO RESPONSE TO PORCINE CIRCOVIRUS TYPE 2 AND PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS CO-INFECTION

作　　者：石坤[1,2] 魏建超[2] 邱亚峰[2] 张克龙[2] 李宗瑞[2] 刘茜倩[2] 李蓓蓓[2] 刘珂[2] 邵东华[2] 陈立志[1] 马志永[2] 

机构地区：[1]中国农业科学院特产研究所,长春130117 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2016年第3期16-20,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点基础研究发展计划(2014CB542703)

摘　　要：为了探讨猪圆环病毒2型(Porcine circovirus type 2,PCV2)与猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)共感染后调控炎症反应的机制,选用稳定表达CD163的PAM细胞系3D4/21,分别设PCV2、PRRSV、PCV2+PRRSV(接种PCV2后12 h接种PRRSV)共感染组和对照组,利用间接免疫荧光检测(indirect immuno fluorescene assay,IFA)方法检测PCV2和PRRSV在PAM-CD163上的阳性率,采用RT-PCR检测IL-6、IL-8、IL-1β、IL-10表达量的动态变化。结果显示PCV2和PRRSV均能感染PAM-CD163;PCV2单独感染PAM-CD163,早期促进了IL-6、IL-8、IL-1β的表达,晚期促进IL-10表达;PRRSV单独感染PAM-CD163能在晚期促进IL-1β、IL-10的表达;与PCV2单独感染PAM-CD163相比,PCV2+PRRSV共感染促进了IL-10表达并且持续增加;与PRRSV单独感染PAM-CD163相比,PCV2+PRRSV共感染促使IL-6、IL-8、IL-1β的表达量较早出现增加,并且持续增加。可见两种病毒之间存在一定的相互作用,共同调节机体免疫系统。本研究结果有助于解析PRRSV与PCV2共感染调控炎症反应的机理。This study was to determine pathogenesis of co-infection of Porcine circovirus type 2 （PCV2） and Porcine reproductive and respiratory syndrome virus （PRRSV） in vitro. A cell line stably expressing CD163 （designated PAM-CD163） were infected with PCV2, PRRSV and PCV2＋PRRSV. The changes of mRNAs of cytokines （IL-6, IL-8, IL-1 D, IL-10） in PAM-CD 163 were analyzed using a realtime fluorescent quantitative PCR （real-time FQ-PCR） and indirect immunofluorescence assay （IFA）. The results showed that both PCV2 and PRRSV could replicate in PAM-CD163. When PAM-CD163 was infected with PCV2 alone, the expression of IL-6, IL-8, IL-I[3 increased at early stage, and IL-10 increased at later stage. While in PAM-CD163 infected with PRRSV, the expression levels of IL-1β,IL-10 were promoted in advanced stage. IL-6, IL-8 and IL-1β in PAM-CD163 began to grow under PCV2＋PRRSV co-infection ahead of that under PCV2 infection, and the increase continued. The findings suggested there were interaction between PCV2 and PRRSV in regulating the immune system.

关 键 词：猪圆环病毒2型 猪繁殖与呼吸综合征病毒 共感染 PAM-CD163 细胞因子 

分 类 号：S852.659.6[农业科学—基础兽医学]