禽致病性大肠杆菌Ⅵ型分泌系统2evfC基因缺失株的构建及其生物学特性分析  

CONSTRUTION AND BIOLOGICAL CHARACTERISTICS ANALYSIS OF TYPE VI SECRETION SYSTEM 2 CORE COMPONENT EVFC MUTANT IN AVIAN PATHOGENIC ESCHERICHIA COLI

作　　者：王栋[1,2] 王少辉[2] 孟庆美[2] 刘新[2] 许漩 杨登辉[2] 韩先干[2] 丁铲[2] 张焕荣 于圣青[2] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2016年第3期21-26,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金项目(31572523;31370045);公益性农业(科研)专项项目(201303044);上海市科技兴农重点攻关项目(沪农科攻字(2015)第1-9号)

摘　　要：为了分析VI型分泌系统2(Type VI secretion system2,T6SS2)核心组分Evf C对禽致病性大肠杆菌(Avian pathogenic Escherichia coli,APEC)生物学特性及致病性的影响,本研究采用Red同源重组系统构建APEC evf C基因缺失株,并利用低拷贝质粒p STV28构建互补株。然后比较分析野生株、缺失株与互补株的生长曲线、运动性、生物被膜形成能力、黏附侵袭能力、胞内存活能力、动物致病力等生物学特性差异。结果表明,evf C基因缺失不影响APEC的生长速度、运动性、生物被膜形成能力、黏附侵袭能力。然而,缺失evf C可导致APEC在HD-11胞内存活能力及对雏鸭的致病力降低。本研究为阐述APEC T6SS2的致病作用提供了参考依据。To determine the roles of type VI secretion system 2 core component EvfC in Avian pathogenic Escherichia coli （APEC）, we constructed the evfC gene mutant strain and complementary strain of APEC DE719 by the Red recombination system and low-copy plasmid pSTV28. Then the growth curve, motility, biofilm formation, adhesion and invasion capacity, intracellular survival capacity and virulence to duck of wild-type strain, mutant strain and complementary strain were compared and analyzed. The results showed that inactivation of evfC gene did not affect the growth, motility capacities, biofilm formation, nor the adhesion and invasion capacities of APEC. However, the evfC gene mutant showed significantly decreased survival capacity and attenuated virulence in ducks. These study would help us to understand the pathogenicity T6SS 2 in APEC.

关 键 词：禽致病性大肠杆菌 Ⅵ型分泌系统2 evfC基因 缺失 

分 类 号：S852.612[农业科学—基础兽医学]