HPLC法测定盐酸多西环素缓释药线中盐酸多西环素的含量  

The Content of Doxycycline Hydrochloride in Doxycycline Hydrochloride Sustained-Release Thread by HPLC

作　　者：胡萍[1] 徐晓栋[1] 任琦[1] 

机构地区：[1]信阳职业技术学院附属医院药剂科,河南信阳464000

出　　处：《中国继续医学教育》2016年第23期134-135,共2页China Continuing Medical Education

摘　　要：目的探讨HPLC法测定盐酸多西环素缓释药线中盐酸多西环素含量。方法盐酸多西环素含量测定应用HPLC法,采用Alltima C 18柱,草酸铵溶液0.05 mol/L：二甲基甲酰胺：磷酸氢二铵溶液0.2mol/L（65：30：5）为流动相,监测波长为280 mm。结果 48-489 mg/L,线性良好,A=43 765 C＋562 000,r=0.999 6,回收率平均为98.35%,RSD为1.30%。结论 HPLC法操作简单,检测结果准确,可作为质量标准检测方法。Objective To explore the determination of doxycycline hydrochloride doxycycline hydrochloride sustained-release thread HPLC. Methods Doxycycline hydrochloride content determination by HPLC, using Alltima C 18 column and 0.05 mol / L ammonium oxalate solution：dimethyl formamide： diammonium hydrogen phosphate solution 0.2 mol / L（65：30：5）as mobile phase, wavelength monitoring for 280 mm. Results 48~489 mg/L, linearity was good, A=43 765 C＋562 000, r=0.999 6, the average recovery rate was 98.35%, RSD was 1.30%. Conclusion HPLC method is simple and accurate, and can be used as a quality standard for testing.

关 键 词：盐酸多西环素 HPLC法 含量 高效液相色谱 

分 类 号：R927[医药卫生—药学]